Detailed Information on Publication Record
2021
Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b
BARTÁKOVÁ, Anna, Tibor STRAČINA, Eva OPATŘILOVÁ and Marie NOVÁKOVÁBasic information
Original name
Guinea Pig ECG Changes under the Effect of New Drug Candidate TP28b
Name in Czech
Změny EKG u morčete pod vlivem nového farmaka TP28b
Authors
BARTÁKOVÁ, Anna (203 Czech Republic, guarantor, belonging to the institution), Tibor STRAČINA (703 Slovakia, belonging to the institution), Eva OPATŘILOVÁ (203 Czech Republic, belonging to the institution) and Marie NOVÁKOVÁ (203 Czech Republic, belonging to the institution)
Edition
Neuveden, Computing in Cardiology 2021, p. 1-4, 4 pp. 2021
Publisher
IEEE
Other information
Language
English
Type of outcome
Stať ve sborníku
Field of Study
30105 Physiology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Publication form
electronic version available online
References:
RIV identification code
RIV/00216224:14110/21:00122659
Organization unit
Faculty of Medicine
ISBN
978-1-6654-7916-5
ISSN
UT WoS
000821955000100
Keywords (in Czech)
EKG; morče; testování látek
Keywords in English
ECG; guinea pig; drug testing
Tags
Tags
International impact, Reviewed
Změněno: 27/6/2024 10:54, Mgr. Michal Petr
V originále
Anesthetized guinea pig represents a valuable model for evaluation of arrhythmogenic potential of various drugs. Aim of this study was to evaluate arrhythmogenic potential of the TP28b, a new drug candidate with possible beta-adrenergic action. Guinea pigs were anesthetized by isoflurane and jugular vein was cannulated. ECG was recorded by needle electrodes fixed subcutaneously on the chest. Rectal probe was used for continual monitoring of body temperature. Animals were divided into positive control, negative control and test group. The experiment consisted of stabilization, 3 phases of tested drug or vehiculum administration by continual i.v. infusion and recovery. The ECG was recorded and analysed using LabChart 8 Pro software. The heart rate, PR intervals, QRS duration, and QT intervals were measured. QT was corrected according to Framingham study. Our experimental model proved to be stable during the whole experiment since the HR, PR interval, QRS interval, and QTc do not change in negative control in all phases. Esmolol administration led to HR decrease, PQ interval and QRS duration prolongation, whereas only small variations of QTc were detected. Our model proved to be valuable and suitable for testing of newly synthetized drugs with cardiovascular effects.
In Czech
Anesthetized guinea pig represents a valuable model for evaluation of arrhythmogenic potential of various drugs. Aim of this study was to evaluate arrhythmogenic potential of the TP28b, a new drug candidate with possible beta-adrenergic action. Guinea pigs were anesthetized by isoflurane and jugular vein was cannulated. ECG was recorded by needle electrodes fixed subcutaneously on the chest. Rectal probe was used for continual monitoring of body temperature. Animals were divided into positive control, negative control and test group. The experiment consisted of stabilization, 3 phases of tested drug or vehiculum administration by continual i.v. infusion and recovery. The ECG was recorded and analysed using LabChart 8 Pro software. The heart rate, PR intervals, QRS duration, and QT intervals were measured. QT was corrected according to Framingham study. Our experimental model proved to be stable during the whole experiment since the HR, PR interval, QRS interval, and QTc do not change in negative control in all phases. Esmolol administration led to HR decrease, PQ interval and QRS duration prolongation, whereas only small variations of QTc were detected. Our model proved to be valuable and suitable for testing of newly synthetized drugs with cardiovascular effects.
Links
| MUNI/A/1246/2020, interní kód MU |
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| MUNI/IGA/1098/2020, interní kód MU |
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