Detailed Information on Publication Record
2021
De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species
BENOVICS, Michal, Lenka GETTOVÁ and Andrea VETEŠNÍKOVÁ ŠIMKOVÁBasic information
Original name
De novo developed microsatellite markers for monogeneans and their application to study population genetics of generalist Dactylogyrus species
Authors
BENOVICS, Michal (703 Slovakia, belonging to the institution), Lenka GETTOVÁ (703 Slovakia, belonging to the institution) and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
26th Helminthological Days, 2021
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
10600 1.6 Biological sciences
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/21:00119293
Organization unit
Faculty of Science
ISBN
978-80-906644-9-4
Keywords in English
Monogenea; Dactylogyrus; Leuciscidae; microsatellites; phylogeography; genetic diversity
Změněno: 22/11/2021 09:11, Mgr. Michal Benovics, Ph.D.
Abstract
V originále
Over the last few decades, genetic markers have been intensively developed to study the population genetics in a wide range of organisms; however, they are weakly applied in parasites' population studies. Microsatellite markers (together with mitochondrial DNA) are considered as a golden standard for population genetic studies. These highly polymorphic short tandem repeats are due to their unique characteristics (e.g., high allelic variance, codominance, and Mendelian inheritance) usually applied to infer gene flow rate, hybridization, or mating patterns on the intraand interpopulation levels. Even though one set of such microsatellite markers was already developed for the monogeneans (more specifically for the Gyrodactylus), they were not previously applied to assess the population structure and diversity of these ectoparasitic helminths. In the present study, we de novo developed set of 24 microsatellite markers and used them to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae parasitizing cyprinoid fish. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans, suggesting that this region might represent the centre of diversification of Dactylogyrus species in Europe, from where Dactylogyrus parasites expanded to the north of Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier to gene flow, even for generalist parasite species.
Links
| GA20-13539S, research and development project |
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