Photon-upconversion nanoparticles as a background-free label in lateral flow immunoassays

MACHÁČOVÁ, Eliška, Julian BRANDMEIER, Dorota SKLENÁROVÁ, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA. Photon-upconversion nanoparticles as a background-free label in lateral flow immunoassays. In NANOCON 2021. 2021. ISBN 978-80-88365-00-6.

Basic information

• Original name: Photon-upconversion nanoparticles as a background-free label in lateral flow immunoassays
• Authors: MACHÁČOVÁ, Eliška, Julian BRANDMEIER, Dorota SKLENÁROVÁ, Hans-Heiner GORRIS, Petr SKLÁDAL and Zdeněk FARKA.
• Edition: NANOCON 2021, 2021.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10406 Analytical chemistry
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• ISBN: 978-80-88365-00-6
• Tags: International impact

Abstract

Sensitive detection of clinically important analytes is critical for early disease diagnosis and initiation of treatment. Immunochemical assays are widely used to determine the concentration of analytes in complex samples due to the high specificity provided by antibodies. Lateral flow immunoassays (LFIAs) belong among the most popular forms of immunoassays. They are based on nitrocellulose membranes, and the most significant advantage of this approach is that there is no need for laboratory equipment to perform the assay. Most LFIAs are based on gold nanoparticles that allow naked-eye readout. However, the recent progress in nanotechnology provided various other nanomaterials that can be used as alternative labels. Our work focused on photon-upconversion nanoparticles (UCNPs), which exhibit anti-Stokes luminescence, meaning they can be excited by the NIR laser and detected in the Vis region without optical background interference. In our proof-of-principle assay, we detected biotinylated bovine serum albumin using streptavidin-coated UCNPs. The testing of different nitrocellulose membranes revealed that FF120HP Plus Thick provides the highest signal-to-background ratio, making it the most suitable for the development of the final LFIA kit. We have also highlighted the necessity of suitable blocking conditions to prevent non-specific binding; the best results were achieved with 50% SuperBlock with 0,05% Tween 20. The signal-to-background ratio of the optimized assay reached up to 290, demonstrating the potential of UCNPs as labels in LFIAs.

Links

• GA21-03156S, research and development project: Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

• LM2018127, large research infrastructures: Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• LM2018127, large research infrastructures: Name: Czech Infrastructure for Integrative Structural Biology (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

• LQ1601, research and development project: Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

• LTAB19011, research and development project: Name: Luminiscenční imunostanovení jako citlivý nástroj pro diagnostiku nemocí včel

Investor: Ministry of Education, Youth and Sports of the CR, Sensitive bioanalytical tools for the surveillance of honey bee diseases, INTER-ACTION