Xeno- and feeder-free derivation of two sex-discordant sibling
lines of human embryonic stem cells

J 2021

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL, Irena KOUTNÁ et. al.

Basic information

Original name

Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells

Authors

SOURALOVÁ, Tereza (203 Czech Republic, belonging to the institution), Zuzana HOLUBCOVÁ (203 Czech Republic, belonging to the institution), Drahomíra KYJOVSKÁ (203 Czech Republic), Aleš HAMPL (203 Czech Republic, belonging to the institution) and Irena KOUTNÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Stem Cell Research, Amsterdam, Elsevier, 2021, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.587

RIV identification code

RIV/00216224:14110/21:00120158

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2021.102574

UT WoS

000712140900005

Keywords in English

human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines

Abstract

V originále

Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.

Links

MUNI/A/1689/2020, interní kód MU

Name: Zdroje pro tkáňové inženýrství 11 (Acronym: TissueEng 11)

Investor: Masaryk University

NV18-08-00412, research and development project

Name: Lidské embryonální kmenové buňky pro klinické aplikace: derivace a charakterizace

Investor: Ministry of Health of the CR

Citovat

SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL and Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. Stem Cell Research. Amsterdam: Elsevier, 2021, vol. 57, December 2021, p. 1-4. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2021.102574.

@article{1799997,
   author = {Souralová, Tereza and Holubcová, Zuzana and Kyjovská, Drahomíra and Hampl, Aleš and Koutná, Irena},
   article_location = {Amsterdam},
   article_number = {December 2021},
   doi = {http://dx.doi.org/10.1016/j.scr.2021.102574},
   keywords = {human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem Cell Research},
   title = {Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells},
   url = {https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub},
   volume = {57},
   year = {2021}
}

TY  - JOUR
ID  - 1799997
AU  - Souralová, Tereza - Holubcová, Zuzana - Kyjovská, Drahomíra - Hampl, Aleš - Koutná, Irena
PY  - 2021
TI  - Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells
JF  - Stem Cell Research
VL  - 57
IS  - December 2021
SP  - 1-4
EP  - 1-4
PB  - Elsevier
SN  - 18735061
KW  - human embryonic stem cells
KW  - Xeno- and feeder-free derivation
KW  - two sex-discordant sibling lines
UR  - https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub
N2  - Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies.
ER  -

SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL and Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. \textit{Stem Cell Research}. Amsterdam: Elsevier, 2021, vol.~57, December 2021, p.~1-4. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2021.102574.

Detailed Information on Publication Record