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@article{1799997, author = {Souralová, Tereza and Holubcová, Zuzana and Kyjovská, Drahomíra and Hampl, Aleš and Koutná, Irena}, article_location = {Amsterdam}, article_number = {December 2021}, doi = {http://dx.doi.org/10.1016/j.scr.2021.102574}, keywords = {human embryonic stem cells; Xeno- and feeder-free derivation; two sex-discordant sibling lines}, language = {eng}, issn = {1873-5061}, journal = {Stem Cell Research}, title = {Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells}, url = {https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub}, volume = {57}, year = {2021} }
TY - JOUR ID - 1799997 AU - Souralová, Tereza - Holubcová, Zuzana - Kyjovská, Drahomíra - Hampl, Aleš - Koutná, Irena PY - 2021 TI - Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells JF - Stem Cell Research VL - 57 IS - December 2021 SP - 1-4 EP - 1-4 PB - Elsevier SN - 18735061 KW - human embryonic stem cells KW - Xeno- and feeder-free derivation KW - two sex-discordant sibling lines UR - https://www.sciencedirect.com/science/article/pii/S1873506121004219?via%3Dihub N2 - Human embryonic stem cells (hESCs) represent a virtually unlimited source of cells suitable for a variety of biomedical applications. However, a diminishing allogeneic background and undefined culture conditions are essential for developing robust and replicable protocols for differentiation experiments, disease modeling, and drug testing. Therefore, here we report the generation of the two sex-discordant sibling hESC lines, MUNIe008-A and MUNIe009-A, using the mechanical biopsy of vitrified-thawed embryos under xeno- and feeder-free conditions. The presented approach is applicable for deriving high-quality clinical-grade hESC lines for cell replacement therapies. ER -
SOURALOVÁ, Tereza, Zuzana HOLUBCOVÁ, Drahomíra KYJOVSKÁ, Aleš HAMPL and Irena KOUTNÁ. Xeno- and feeder-free derivation of two sex-discordant sibling lines of human embryonic stem cells. \textit{Stem Cell Research}. Amsterdam: Elsevier, 2021, vol.~57, December 2021, p.~1-4. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2021.102574.
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