ŠÁMALOVÁ, Markéta and Ian MOORE. The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa). BMC Plant Biology. London: BioMed Central Ltd., 2021, vol. 21, No 1, p. 461-475. ISSN 1471-2229. Available from: https://dx.doi.org/10.1186/s12870-021-03241-w.
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Basic information
Original name The steroid-inducible pOp6/LhGR gene expression system is fast, sensitive and does not cause plant growth defects in rice (Oryza sativa)
Authors ŠÁMALOVÁ, Markéta (203 Czech Republic, guarantor, belonging to the institution) and Ian MOORE.
Edition BMC Plant Biology, London, BioMed Central Ltd. 2021, 1471-2229.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10611 Plant sciences, botany
Country of publisher United Kingdom of Great Britain and Northern Ireland
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 5.260
RIV identification code RIV/00216224:14310/21:00122699
Organization unit Faculty of Science
Doi http://dx.doi.org/10.1186/s12870-021-03241-w
UT WoS 000705202600001
Keywords in English Dexamethasone; Glucocorticoid; Chemically inducible gene expression; GVG; XVE; Promotor; Monocots
Tags rivok
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marie Šípková, DiS., učo 437722. Changed: 1/11/2021 10:47.
Abstract
Inducible systems for transgene expression activated by a chemical inducer or an inducer of non-plant origin are desirable tools for both basic plant research and biotechnology. Although, the technology has been widely exploited in dicotyledonous model plants such as Arabidopsis, it has not been optimised for use with the monocotyledonous model species, namely rice. We have adapted the dexamethasone-inducible pOp6/LhGR system for rice and the results indicated that it is fast, sensitive and tightly regulated, with high levels of induction that remain stable over several generations. Most importantly, we have shown that the system does not cause negative growth defects in vitro or in soil grown plants. Interestingly in the process of testing, we found that another steroid, triamcinolone acetonide, is a more potent inducer in rice than dexamethasone. We present serious considerations for the construct design to avoid undesirable effects caused by the system in plants, leakiness and possible silencing, as well as simple steps to maximize translation efficiency of a gene of interest. Finally, we compare the performance of the pOp6/LhGR system with other chemically inducible systems tested in rice in terms of the properties of an ideal inducible system.
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