| ŠENOVSKÁ, Anna, Eva DROZDOVÁ, Kristýna BRZOBOHATÁ, Eva CHOCHOLOVÁ, Dana FIALOVÁ a Jaromír ŠMERDA. Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing. Journal of Archaeological Science: Reports. Elsevier Ltd., roč. 40, December, s. 1-5. ISSN 2352-409X. doi:10.1016/j.jasrep.2021.103216. 2021. |
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@article{1800817,
author = {Šenovská, Anna and Drozdová, Eva and Brzobohatá, Kristýna and Chocholová, Eva and Fialová, Dana and Šmerda, Jaromír},
article_number = {December},
doi = {http://dx.doi.org/10.1016/j.jasrep.2021.103216},
keywords = {ancient DNA; mitochondrial DNA; Sanger sequencing; hypervariable region},
language = {eng},
issn = {2352-409X},
journal = {Journal of Archaeological Science: Reports},
title = {Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing},
url = {https://doi.org/10.1016/j.jasrep.2021.103216},
volume = {40},
year = {2021}
}
TY - JOUR
ID - 1800817
AU - Šenovská, Anna - Drozdová, Eva - Brzobohatá, Kristýna - Chocholová, Eva - Fialová, Dana - Šmerda, Jaromír
PY - 2021
TI - Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing
JF - Journal of Archaeological Science: Reports
VL - 40
IS - December
SP - 1-5
EP - 1-5
PB - Elsevier Ltd.
SN - 2352409X
KW - ancient DNA
KW - mitochondrial DNA
KW - Sanger sequencing
KW - hypervariable region
UR - https://doi.org/10.1016/j.jasrep.2021.103216
N2 - Working with ancient DNA is challenging and requires an authentication step. We analyzed eleven badly preserved samples from the Nitra culture with the influence of Únětice culture dated to the 4000 BP Bronze Age. We present estimated mitochondrial haplogroups of four samples based on Sanger sequencing of HVR1 and a part of HVR2. Sanger sequencing has strong limitations in haplogroup prediction, especially when working with ancient DNA. However, Sanger sequencing in this study is featured as a control step to provide us with quality results of mitochondrial DNA and as a screening of present haplogroups. DNA quality estimation is further supported by sample concentration and fragment analysis. The presence of mitochondrial DNA is confirmed by PCR. However, the Sanger sequencing of mitochondrial DNA can confirm its presence in good quality for further sequencing. Therefore, only suitable samples were selected for further high-throughput sequencing of the whole mitogenome.
ER -
ŠENOVSKÁ, Anna, Eva DROZDOVÁ, Kristýna BRZOBOHATÁ, Eva CHOCHOLOVÁ, Dana FIALOVÁ a Jaromír ŠMERDA. Sanger sequencing of mitochondrial hypervariable region of ancient samples for DNA authentication and screening before high-throughput sequencing. \textit{Journal of Archaeological Science: Reports}. Elsevier Ltd., roč.~40, December, s.~1-5. ISSN~2352-409X. doi:10.1016/j.jasrep.2021.103216. 2021.
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