J 2021

Generation of six human iPSC lines from patients with a familial Alzheimer's disease (n=3) and sex- and age-matched healthy controls (n=3)

RAŠKA, Jan, Hana HŘÍBKOVÁ, Hana KLÍMOVÁ, Veronika FEDOROVÁ, Martin BARÁK et. al.

Basic information

Original name

Generation of six human iPSC lines from patients with a familial Alzheimer's disease (n=3) and sex- and age-matched healthy controls (n=3)

Authors

RAŠKA, Jan (203 Czech Republic, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Hana KLÍMOVÁ (203 Czech Republic, belonging to the institution), Veronika FEDOROVÁ (203 Czech Republic, belonging to the institution), Martin BARÁK (203 Czech Republic, belonging to the institution), Tomáš BÁRTA (203 Czech Republic, belonging to the institution), Veronika POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Simona VOCHYÁNOVÁ (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution) and Dáša BOHAČIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Stem cell research, Amsterdam, Elsevier, 2021, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.587

RIV identification code

RIV/00216224:14110/21:00120161

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2021.102379

UT WoS

000656247000011

Keywords in English

six human iPSC lines; Alzheimer's disease; healthy controls

Tags

14110517, CF CELLIM, rivok

Tags

International impact, Reviewed
Změněno: 21/2/2022 10:37, Mgr. Tereza Miškechová

Abstract

V originále

Human induced pluripotent stem cell (iPSC) lines were generated from primary human fibroblasts isolated from three patients with a familial form of Alzheimer's disease (AD) and three healthy control individuals. Two AD-iPSC lines carry a PSEN1 mutation A246E; the third cell line carries a PSEN2 mutation N141I. The fibroblasts were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.

Links

EF17_043/0009632, research and development project
Name: CETOCOEN Excellence
LM2018121, research and development project
Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)
Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI
LM2018129, research and development project
Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/A/1382/2019, interní kód MU
Name: Zdroje pro tkáňové inženýrství 10 (Acronym: TissueEng 10)
Investor: Masaryk University, Category A
MUNI/A/1689/2020, interní kód MU
Name: Zdroje pro tkáňové inženýrství 11 (Acronym: TissueEng 11)
Investor: Masaryk University
MUNI/G/1131/2017, interní kód MU
Name: Transformative stem cell-based model of Alzheimer’s disease and advanced analytics to study the role of membrane lipids in the pathogenesis
Investor: Masaryk University, INTERDISCIPLINARY - Interdisciplinary research projects
MUNI/R/1697/2020, interní kód MU
Name: Career Restart - Stem Cell-Based Models of Neurodegeneration
Investor: Masaryk University, CAREER RESTART
NV19-08-00472, research and development project
Name: Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí (Acronym: BioMAD)
Investor: Ministry of Health of the CR
ROZV/23/LF19/2019, interní kód MU
Name: Využití in vitro kultivovaných cerebrálních organoidů pro modelování patogeneze Alzheimerovy choroby
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
ROZV/28/LF22/2020, interní kód MU
Name: ln vitro modelováni Alzheimerovy choroby pomoci nově derivovaných indukovaných plunpotentnich kmenových buněk z pacientu se spontánní formou onemocnění.
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
Displayed: 3/11/2024 13:31