BARTOS, M., František SIEGL, Alena KOPKOVÁ, Lenka RADOVÁ, Jan OPPELT, Marek VEČEŘA, Tomáš KAZDA, Radim JANČÁLEK, Michal HENDRYCH, Markéta HERMANOVÁ, P. KASPAROVA, Z. PLESKACOVA, Václav VYBÍHAL, P. FADRUS, M. SMRCKA, R. LAKOMY, R. LIPINA, T. CESAK, Ondřej SLABÝ and Jiří ŠÁNA. Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro. Frontiers in Oncology. Lausanne: Frontiers Media S.A., 2021, vol. 11, AUG, p. 1-11. ISSN 2234-943X. Available from: https://dx.doi.org/10.3389/fonc.2021.707017. |
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@article{1804764, author = {Bartos, M. and Siegl, František and Kopková, Alena and Radová, Lenka and Oppelt, Jan and Večeřa, Marek and Kazda, Tomáš and Jančálek, Radim and Hendrych, Michal and Hermanová, Markéta and Kasparova, P. and Pleskacova, Z. and Vybíhal, Václav and Fadrus, P. and Smrcka, M. and Lakomy, R. and Lipina, R. and Cesak, T. and Slabý, Ondřej and Šána, Jiří}, article_location = {Lausanne}, article_number = {AUG}, doi = {http://dx.doi.org/10.3389/fonc.2021.707017}, keywords = {glioblastoma; PIWI-interacting RNA; piRNA; diagnosis; prognosis}, language = {eng}, issn = {2234-943X}, journal = {Frontiers in Oncology}, title = {Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro}, url = {https://www.frontiersin.org/articles/10.3389/fonc.2021.707017/full}, volume = {11}, year = {2021} }
TY - JOUR ID - 1804764 AU - Bartos, M. - Siegl, František - Kopková, Alena - Radová, Lenka - Oppelt, Jan - Večeřa, Marek - Kazda, Tomáš - Jančálek, Radim - Hendrych, Michal - Hermanová, Markéta - Kasparova, P. - Pleskacova, Z. - Vybíhal, Václav - Fadrus, P. - Smrcka, M. - Lakomy, R. - Lipina, R. - Cesak, T. - Slabý, Ondřej - Šána, Jiří PY - 2021 TI - Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro JF - Frontiers in Oncology VL - 11 IS - AUG SP - 1-11 EP - 1-11 PB - Frontiers Media S.A. SN - 2234943X KW - glioblastoma KW - PIWI-interacting RNA KW - piRNA KW - diagnosis KW - prognosis UR - https://www.frontiersin.org/articles/10.3389/fonc.2021.707017/full N2 - Glioblastoma (GBM) is the most frequently occurring primary malignant brain tumor of astrocytic origin. To change poor prognosis, it is necessary to deeply understand the molecular mechanisms of gliomagenesis and identify new potential biomarkers and therapeutic targets. PIWI-interacting RNAs (piRNAs) help in maintaining genome stability, and their deregulation has already been observed in many tumors. Recent studies suggest that these molecules could also play an important role in the glioma biology. To determine GBM-associated piRNAs, we performed small RNA sequencing analysis in the discovery set of 19 GBM and 11 non-tumor brain samples followed by TaqMan qRT-PCR analyses in the independent set of 77 GBM and 23 non-tumor patients. Obtained data were subsequently bioinformatically analyzed. Small RNA sequencing revealed 58 significantly deregulated piRNA molecules in GBM samples in comparison with non-tumor brain tissues. Deregulation of piR-1849, piR-9491, piR-12487, and piR-12488 was successfully confirmed in the independent groups of patients and controls (all p < 0.0001), and piR-9491 and piR-12488 reduced GBM cells' ability to form colonies in vitro. In addition, piR-23231 was significantly associated with the overall survival of the GBM patients treated with Stupp regimen (p = 0.007). Our results suggest that piRNAs could be a novel promising diagnostic and prognostic biomarker in GBM potentially playing important roles in gliomagenesis. ER -
BARTOS, M., František SIEGL, Alena KOPKOVÁ, Lenka RADOVÁ, Jan OPPELT, Marek VEČEŘA, Tomáš KAZDA, Radim JANČÁLEK, Michal HENDRYCH, Markéta HERMANOVÁ, P. KASPAROVA, Z. PLESKACOVA, Václav VYBÍHAL, P. FADRUS, M. SMRCKA, R. LAKOMY, R. LIPINA, T. CESAK, Ondřej SLABÝ and Jiří ŠÁNA. Small RNA Sequencing Identifies PIWI-Interacting RNAs Deregulated in Glioblastoma-piR-9491 and piR-12488 Reduce Tumor Cell Colonies In Vitro. \textit{Frontiers in Oncology}. Lausanne: Frontiers Media S.A., 2021, vol.~11, AUG, p.~1-11. ISSN~2234-943X. Available from: https://dx.doi.org/10.3389/fonc.2021.707017.
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