Escherichia coli Strains Producing Selected Bacteriocins
Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under
both In Vitro and In Vivo Conditions

J 2021

Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions

HRALA, Matěj, Juraj BOSÁK, Lenka MICENKOVÁ, Jitka KŘENOVÁ, Matej LEXA et. al.

Basic information

Original name

Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions

Authors

HRALA, Matěj (203 Czech Republic, belonging to the institution), Juraj BOSÁK (703 Slovakia, belonging to the institution), Lenka MICENKOVÁ (703 Slovakia, belonging to the institution), Jitka KŘENOVÁ (203 Czech Republic, belonging to the institution), Matej LEXA (703 Slovakia, belonging to the institution), Viktória PIRKOVÁ (703 Slovakia, belonging to the institution), Zuzana TOMASTIKOVA (203 Czech Republic), Ivana KOLÁČKOVÁ (203 Czech Republic) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

Applied and Environmental Microbiology, WASHINGTON, DC (USA), AMER SOC MICROBIOLOGY, 2021, 0099-2240

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.005

RIV identification code

RIV/00216224:14110/21:00123081

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1128/AEM.03121-20

UT WoS

000693249500022

Keywords in English

probiotic; Escherichia; E. coli; bacteriocin; pig; ETEC; STEC

Abstract

V originále

Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n =277) as well as porcine commensal strains (n = 188) were tested for their susceptibilities to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro conditions, the set of pathogenic E. coli strains was found to be significantly more susceptible to the majority of tested bacteriocins than commensal E. coli. Based on the production of bacteriocins with specific activity against pathogens, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decreased the severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers on the last day of in vivo experimentation. While the extents of the probiotic effect were different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects, suggesting synergy between the tested E. coli strains. IMPORTANCE Increasing levels of antibiotic resistance among bacteria also increase the need for alternatives to conventional antibiotic treatment. Pathogenic Escherichia coli represents a major diarrheic infectious agent of piglets in their postweaning period; however, available measures to control these infections are limited. This study describes three novel E. coli strains producing antimicrobial compounds (bacteriocins) that actively inhibit a majority of toxigenic E. coli strains. The beneficial effect of three potentially probiotic E. coli strains was demonstrated under both in vitro and in vivo conditions. The novel probiotic candidates may be used as prophylaxis during piglets' postweaning period to overcome common infections caused by E. coli.

Links

EF16_013/0001761, research and development project

Name: RECETOX RI

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

MUNI/A/1325/2020, interní kód MU

Name: Biomedicínské vědy

Investor: Masaryk University

ROZV/23/LF13/2019, interní kód MU

Name: Terapeutický potenciál bakteriocinogenních probiotik v léčbě kolibakterióz

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

ROZV/28/LF15/2020, interní kód MU

Name: Role bakteriocinů u infekcí močových cest

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

HRALA, Matěj, Juraj BOSÁK, Lenka MICENKOVÁ, Jitka KŘENOVÁ, Matej LEXA, Viktória PIRKOVÁ, Zuzana TOMASTIKOVA, Ivana KOLÁČKOVÁ and David ŠMAJS. Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions. Applied and Environmental Microbiology. WASHINGTON, DC (USA): AMER SOC MICROBIOLOGY, 2021, vol. 87, No 14, p. 1-12. ISSN 0099-2240. Available from: https://dx.doi.org/10.1128/AEM.03121-20.

@article{1806698,
   author = {Hrala, Matěj and Bosák, Juraj and Micenková, Lenka and Křenová, Jitka and Lexa, Matej and Pirková, Viktória and Tomastikova, Zuzana and Koláčková, Ivana and Šmajs, David},
   article_location = {WASHINGTON, DC (USA)},
   article_number = {14},
   doi = {http://dx.doi.org/10.1128/AEM.03121-20},
   keywords = {probiotic; Escherichia; E. coli; bacteriocin; pig; ETEC; STEC},
   language = {eng},
   issn = {0099-2240},
   journal = {Applied and Environmental Microbiology},
   title = {Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions},
   url = {https://journals.asm.org/doi/10.1128/AEM.03121-20},
   volume = {87},
   year = {2021}
}

TY  - JOUR
ID  - 1806698
AU  - Hrala, Matěj - Bosák, Juraj - Micenková, Lenka - Křenová, Jitka - Lexa, Matej - Pirková, Viktória - Tomastikova, Zuzana - Koláčková, Ivana - Šmajs, David
PY  - 2021
TI  - Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions
JF  - Applied and Environmental Microbiology
VL  - 87
IS  - 14
SP  - 1-12
EP  - 1-12
PB  - AMER SOC MICROBIOLOGY
SN  - 00992240
KW  - probiotic
KW  - Escherichia
KW  - E. coli
KW  - bacteriocin
KW  - pig
KW  - ETEC
KW  - STEC
UR  - https://journals.asm.org/doi/10.1128/AEM.03121-20
N2  - Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) strains are the causative agents of severe foodborne diseases in both humans and animals. In this study, porcine pathogenic E. coli strains (n =277) as well as porcine commensal strains (n = 188) were tested for their susceptibilities to 34 bacteriocin monoproducers to identify the most suitable bacteriocin types inhibiting porcine pathogens. Under in vitro conditions, the set of pathogenic E. coli strains was found to be significantly more susceptible to the majority of tested bacteriocins than commensal E. coli. Based on the production of bacteriocins with specific activity against pathogens, three potentially probiotic commensal E. coli strains of human origin were selected. These strains were found to be able to outcompete ETEC strains expressing F4 or F18 fimbriae in liquid culture and also decreased the severity and duration of diarrhea in piglets during experimental ETEC infection as well as pathogen numbers on the last day of in vivo experimentation. While the extents of the probiotic effect were different for each strain, the cocktail of all three strains showed the most pronounced beneficial effects, suggesting synergy between the tested E. coli strains. IMPORTANCE Increasing levels of antibiotic resistance among bacteria also increase the need for alternatives to conventional antibiotic treatment. Pathogenic Escherichia coli represents a major diarrheic infectious agent of piglets in their postweaning period; however, available measures to control these infections are limited. This study describes three novel E. coli strains producing antimicrobial compounds (bacteriocins) that actively inhibit a majority of toxigenic E. coli strains. The beneficial effect of three potentially probiotic E. coli strains was demonstrated under both in vitro and in vivo conditions. The novel probiotic candidates may be used as prophylaxis during piglets' postweaning period to overcome common infections caused by E. coli.
ER  -

HRALA, Matěj, Juraj BOSÁK, Lenka MICENKOVÁ, Jitka KŘENOVÁ, Matej LEXA, Viktória PIRKOVÁ, Zuzana TOMASTIKOVA, Ivana KOLÁČKOVÁ and David ŠMAJS. Escherichia coli Strains Producing Selected Bacteriocins Inhibit Porcine Enterotoxigenic Escherichia coli (ETEC) under both In Vitro and In Vivo Conditions. \textit{Applied and Environmental Microbiology}. WASHINGTON, DC (USA): AMER SOC MICROBIOLOGY, 2021, vol.~87, No~14, p.~1-12. ISSN~0099-2240. Available from: https://dx.doi.org/10.1128/AEM.03121-20.

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