Enhancement of luminescence signal by deuterated water - Practical implications

KUČERA, Josef, Ondřej PEŠ, Tomáš JANOVIČ, Ctirad HOFR, Lenka KUBINYIOVÁ, Ján TÓTH, Štěpán KÁŇA and Petr TÁBORSKÝ. Enhancement of luminescence signal by deuterated water - Practical implications. Sensors and Actuators B: Chemical. Amsterdam: Elsevier B.V., 2022, vol. 352, No 1, p. 1-8. ISSN 0925-4005. Available from: https://dx.doi.org/10.1016/j.snb.2021.131029.

Basic information

Original name

Enhancement of luminescence signal by deuterated water - Practical implications

Authors

KUČERA, Josef (203 Czech Republic, belonging to the institution), Ondřej PEŠ (203 Czech Republic, belonging to the institution), Tomáš JANOVIČ (203 Czech Republic, belonging to the institution), Ctirad HOFR (203 Czech Republic, belonging to the institution), Lenka KUBINYIOVÁ (203 Czech Republic, belonging to the institution), Ján TÓTH (703 Slovakia, belonging to the institution), Štěpán KÁŇA (203 Czech Republic, belonging to the institution) and Petr TÁBORSKÝ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Sensors and Actuators B: Chemical, Amsterdam, Elsevier B.V. 2022, 0925-4005

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 8.400

RIV identification code

RIV/00216224:14310/22:00119391

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.snb.2021.131029

UT WoS

000721027600003

Keywords in English

Biomolecules; Capillary electrophoresis; Fluorescence correlation spectroscopy; Fluorescence lifetime imaging microscopy; Fluorescence microscopy; Fluorescent probes and labels

Tags

14110512, podil, rivok

Tags

International impact, Reviewed

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Abstract

V originále

The fluorescence signal increase in deuterated water - D2O is relevant to many methods used in chemical and biological research. We performed fluorescence spectroscopy measurements combined with evaluations of fluorescence-based methods to describe and quantify the practical impacts of D2O on chemical and biological samples. Several novel features are observed in fluorescence spectra and life-time decay curves of molecules including fluorescence probes and labels that are used frequently in biomolecular labelling and bioimaging. The observed characteristics suggest that D2O enhances the fluorescence signal due to the exchange of labile hydrogen on a fluorophore for heavier deuteron and the reduction of energy transfer from excited fluorophores to H2O molecules. Furthermore, performed quantifications approve that D2O increases significantly the detection signal of all examined fluorescence-based approaches where the brightness of fluorophores and sample viability are crucial.

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Links

GA19-18226S, research and development project	Name: Kritické interakce neuronového transkripčního faktoru REST se stabilizátorem TRF2: biofyzikální implikace pro návrh léčiv glioblastomu Investor: Czech Science Foundation
LTAUSA19024, research and development project	Name: Role regulace transkripce při chemoterapeutické rezistenci glioblastomu: in vitro a in vivo validace cíle pro molekulární terapii Investor: Ministry of Education, Youth and Sports of the CR, INTER-ACTION
MUNI/A/1167/2019, interní kód MU	Name: Příspěvek (bio)chemických technik ke studiu molekulární podstaty vybraných patologických jevů a onemocnění Investor: Masaryk University, Category A
MUNI/A/1266/2020, interní kód MU	Name: Příspěvek [bio]chemických metod při studiu molekulární podstaty vybraných patologických jevů a onemocnění Investor: Masaryk University
MUNI/A/1421/2019, interní kód MU	Name: Analytické a fyzikálně-chemické metody ve výzkumu geologických, biologických a syntetických materiálů (Acronym: AFCHMAT) Investor: Masaryk University, Category A
MUNI/A/1424/2019, interní kód MU	Name: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2020 Investor: Masaryk University, Category A
Visegrad 51910104, interní kód MU	Name: Visegrad scholarship Ján Tóth Investor: International Visegrad Fund (IVF)