| KUČERA, Josef, Ondřej PEŠ, Tomáš JANOVIČ, Ctirad HOFR, Lenka KUBINYIOVÁ, Ján TÓTH, Štěpán KÁŇA a Petr TÁBORSKÝ. Enhancement of luminescence signal by deuterated water - Practical implications. Sensors and Actuators B: Chemical. Amsterdam: Elsevier B.V., roč. 352, č. 1, s. 1-8. ISSN 0925-4005. doi:10.1016/j.snb.2021.131029. 2022. |
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@article{1806821,
author = {Kučera, Josef and Peš, Ondřej and Janovič, Tomáš and Hofr, Ctirad and Kubinyiová, Lenka and Tóth, Ján and Káňa, Štěpán and Táborský, Petr},
article_location = {Amsterdam},
article_number = {1},
doi = {http://dx.doi.org/10.1016/j.snb.2021.131029},
keywords = {Biomolecules; Capillary electrophoresis; Fluorescence correlation spectroscopy; Fluorescence lifetime imaging microscopy; Fluorescence microscopy; Fluorescent probes and labels},
language = {eng},
issn = {0925-4005},
journal = {Sensors and Actuators B: Chemical},
title = {Enhancement of luminescence signal by deuterated water - Practical implications},
url = {https://www.sciencedirect.com/science/article/pii/S0925400521015975?via%3Dihub},
volume = {352},
year = {2022}
}
TY - JOUR
ID - 1806821
AU - Kučera, Josef - Peš, Ondřej - Janovič, Tomáš - Hofr, Ctirad - Kubinyiová, Lenka - Tóth, Ján - Káňa, Štěpán - Táborský, Petr
PY - 2022
TI - Enhancement of luminescence signal by deuterated water - Practical implications
JF - Sensors and Actuators B: Chemical
VL - 352
IS - 1
SP - 1-8
EP - 1-8
PB - Elsevier B.V.
SN - 09254005
KW - Biomolecules
KW - Capillary electrophoresis
KW - Fluorescence correlation spectroscopy
KW - Fluorescence lifetime imaging microscopy
KW - Fluorescence microscopy
KW - Fluorescent probes and labels
UR - https://www.sciencedirect.com/science/article/pii/S0925400521015975?via%3Dihub
N2 - The fluorescence signal increase in deuterated water - D2O is relevant to many methods used in chemical and biological research. We performed fluorescence spectroscopy measurements combined with evaluations of fluorescence-based methods to describe and quantify the practical impacts of D2O on chemical and biological samples. Several novel features are observed in fluorescence spectra and life-time decay curves of molecules including fluorescence probes and labels that are used frequently in biomolecular labelling and bioimaging. The observed characteristics suggest that D2O enhances the fluorescence signal due to the exchange of labile hydrogen on a fluorophore for heavier deuteron and the reduction of energy transfer from excited fluorophores to H2O molecules. Furthermore, performed quantifications approve that D2O increases significantly the detection signal of all examined fluorescence-based approaches where the brightness of fluorophores and sample viability are crucial.
ER -
KUČERA, Josef, Ondřej PEŠ, Tomáš JANOVIČ, Ctirad HOFR, Lenka KUBINYIOVÁ, Ján TÓTH, Štěpán KÁŇA a Petr TÁBORSKÝ. Enhancement of luminescence signal by deuterated water - Practical implications. \textit{Sensors and Actuators B: Chemical}. Amsterdam: Elsevier B.V., roč.~352, č.~1, s.~1-8. ISSN~0925-4005. doi:10.1016/j.snb.2021.131029. 2022.
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