| REBROŠOVÁ, Katarína, Silvie BERNATOVÁ, Martin ŠILER, Magdalena UHLIROVA, Ota SAMEK, Jan JEŽEK, Veronika HOLÁ, Filip RŮŽIČKA and Pavel ZEMANEK. Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections. Analytica Chimica Acta. Amsterdam: Elsevier, 2022, vol. 1191, January 2022, p. 1-10. ISSN 0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2021.339292. |
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@article{1807738,
author = {Rebrošová, Katarína and Bernatová, Silvie and Šiler, Martin and Uhlirova, Magdalena and Samek, Ota and Ježek, Jan and Holá, Veronika and Růžička, Filip and Zemanek, Pavel},
article_location = {Amsterdam},
article_number = {January 2022},
doi = {http://dx.doi.org/10.1016/j.aca.2021.339292},
keywords = {Raman spectroscopy; Urinary tract infections; Raman tweezers; Optical trapping; Bacteria},
language = {eng},
issn = {0003-2670},
journal = {Analytica Chimica Acta},
title = {Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections},
url = {https://www.sciencedirect.com/science/article/pii/S0003267021011181?via%3Dihub},
volume = {1191},
year = {2022}
}
TY - JOUR
ID - 1807738
AU - Rebrošová, Katarína - Bernatová, Silvie - Šiler, Martin - Uhlirova, Magdalena - Samek, Ota - Ježek, Jan - Holá, Veronika - Růžička, Filip - Zemanek, Pavel
PY - 2022
TI - Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections
JF - Analytica Chimica Acta
VL - 1191
IS - January 2022
SP - 1-10
EP - 1-10
PB - Elsevier
SN - 00032670
KW - Raman spectroscopy
KW - Urinary tract infections
KW - Raman tweezers
KW - Optical trapping
KW - Bacteria
UR - https://www.sciencedirect.com/science/article/pii/S0003267021011181?via%3Dihub
N2 - Urinary tract infections belong to the most common infections in the world. Besides community-acquired infections, nosocomial infections pose a high risk especially for patients having indwelling catheters, undergoing urological surgeries or staying at hospital for prolonged time. They can be often complicated by antimicrobial resistance and/or biofilm formation. Therefore, a rapid diagnostic tool enabling timely identification of a causative agent and its susceptibility to antimicrobials is a need. Raman spectroscopy appears to be a suitable method that allows rapid differentiation among microbes and provides a space for further analyses, such as determination of capability of biofilm formation or antimicrobial susceptibility/resistance in tested strains. Our work here presents a possibility to differ among most common microbes causing urinary tract infections (belonging to 20 species). We tested 254 strains directly from colonies grown on Mueller-Hinton agar plates. The results show that it is possible to distinguish among the tested species using Raman spectroscopy, which proves its great potential for future use in clinical diagnostics. Moreover, we present here a pilot study of a real-time analysis and identification (in less than 10 min) of single microbial cells directly in urine employing optical tweezers combined with Raman spectroscopy.
ER -
REBROŠOVÁ, Katarína, Silvie BERNATOVÁ, Martin ŠILER, Magdalena UHLIROVA, Ota SAMEK, Jan JEŽEK, Veronika HOLÁ, Filip RŮŽIČKA and Pavel ZEMANEK. Raman spectroscopy - a tool for rapid differentiation among microbes causing urinary tract infections. \textit{Analytica Chimica Acta}. Amsterdam: Elsevier, 2022, vol.~1191, January 2022, p.~1-10. ISSN~0003-2670. Available from: https://dx.doi.org/10.1016/j.aca.2021.339292.
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