Detailed Information on Publication Record
2020
NEW CRYOPRESERVATION TECHNOLOGY OF HMSCS: FIRST PRECLINICAL RESULTS USING DMSO-CONTAINING MEDIUM
JANDOVA, M., P. SPONER, D. VOKURKOVA, P. O. BAUER, A. FILIPOVA et. al.Basic information
Original name
NEW CRYOPRESERVATION TECHNOLOGY OF HMSCS: FIRST PRECLINICAL RESULTS USING DMSO-CONTAINING MEDIUM
Authors
JANDOVA, M., P. SPONER, D. VOKURKOVA, P. O. BAUER, A. FILIPOVA, S. FILIP and P. MERICKA
Edition
CryoLetters, LONDON, CRYO LETTERS, 2020, 0143-2044
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30230 Other clinical medicine subjects
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 1.066
UT WoS
000527407900009
Keywords in English
mesenchymal stem cells; advanced therapy medicinal product; dimethyl sulfoxide; cryopreservation
Tags
Změněno: 7/12/2021 13:01, Bc. Hana Vladíková, BBA
Abstract
V originále
BACKGROUND: Human mesenchymal stem cells (hMSCs) have tremendous potential in regenerative medicine, making it desirable to cryopreserve and bank them to increase their access and availability. OBJECTIVE: This research is part of a clinical trial performed on six patients that aimed to use advanced therapy medicinal products (ATMPs) based on hMSCs in patients undergoing repeated total hip replacement. MATERIALS AND METHODS: To compare the characteristics of fresh and frozen hMSCs, we used the trypan blue exclusion test (cell viability), flow cytometry (cell viability and phenotyping), sterility determinations and the clonogenic assay of cell proliferation. RESULTS: Cryopreserved hMSCs showed good quality parameters after thawing in comparison with fresh hMSCs in suspension. When using a medium containing dimethyl sulfoxide (DMSO), the viability was higher than 90% in all cases. The cell purity determined by flow cytometry was also acceptable. CONCLUSION: These initial results show that the prepared cryopreserved ATMP exhibited good viability and phenotype characteristics.
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