Upgrade of The Langendorff Apparatus Using The Infrared
Thermo-Control System and An Intelligent Heater

J 2020

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

SKOPALÍK, Josef, Jiří SEKORA, Martin PEŠL, Markéta BÉBAROVÁ, Olga ŠVECOVÁ et. al.

Basic information

Original name

Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

Authors

SKOPALÍK, Josef (203 Czech Republic, guarantor), Jiří SEKORA (203 Czech Republic), Martin PEŠL (203 Czech Republic, belonging to the institution), Markéta BÉBAROVÁ (203 Czech Republic, belonging to the institution), Olga ŠVECOVÁ (203 Czech Republic, belonging to the institution), Tomáš PARÁK (203 Czech Republic), Vratislav ČMIEL (203 Czech Republic), Ivo PROVAZNÍK (203 Czech Republic), Edita JEKLOVÁ (203 Czech Republic) and Josef MAŠEK (203 Czech Republic)

Edition

Lékař a technika, Praha, Czech Society for Biomedical Engineering and Medical Informatics, 2020, 0301-5491

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14110/20:00123174

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.14311/CTJ.2020.4.03

Keywords in English

Langendorff Apparatus; pig heart; infrared; thermo-control; heater

Abstract

V originále

Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.

Links

MUNI/A/1307/2019, interní kód MU

Name: Kardiovaskulární systém od A do Z (Acronym: KAVASAZ)

Investor: Masaryk University, Category A

Citovat

SKOPALÍK, Josef, Jiří SEKORA, Martin PEŠL, Markéta BÉBAROVÁ, Olga ŠVECOVÁ, Tomáš PARÁK, Vratislav ČMIEL, Ivo PROVAZNÍK, Edita JEKLOVÁ and Josef MAŠEK. Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater. Lékař a technika. Praha: Czech Society for Biomedical Engineering and Medical Informatics, 2020, vol. 50, No 4, p. 137-141. ISSN 0301-5491. Available from: https://dx.doi.org/10.14311/CTJ.2020.4.03.

@article{1808290,
   author = {Skopalík, Josef and Sekora, Jiří and Pešl, Martin and Bébarová, Markéta and Švecová, Olga and Parák, Tomáš and Čmiel, Vratislav and Provazník, Ivo and Jeklová, Edita and Mašek, Josef},
   article_location = {Praha},
   article_number = {4},
   doi = {http://dx.doi.org/10.14311/CTJ.2020.4.03},
   keywords = {Langendorff Apparatus; pig heart; infrared; thermo-control; heater},
   language = {eng},
   issn = {0301-5491},
   journal = {Lékař a technika},
   title = {Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater},
   url = {https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126},
   volume = {50},
   year = {2020}
}

TY  - JOUR
ID  - 1808290
AU  - Skopalík, Josef - Sekora, Jiří - Pešl, Martin - Bébarová, Markéta - Švecová, Olga - Parák, Tomáš - Čmiel, Vratislav - Provazník, Ivo - Jeklová, Edita - Mašek, Josef
PY  - 2020
TI  - Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater
JF  - Lékař a technika
VL  - 50
IS  - 4
SP  - 137-141
EP  - 137-141
PB  - Czech Society for Biomedical Engineering and Medical Informatics
SN  - 03015491
KW  - Langendorff Apparatus
KW  - pig heart
KW  - infrared
KW  - thermo-control
KW  - heater
UR  - https://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126
N2  - Biological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.
ER  -

SKOPALÍK, Josef, Jiří SEKORA, Martin PEŠL, Markéta BÉBAROVÁ, Olga ŠVECOVÁ, Tomáš PARÁK, Vratislav ČMIEL, Ivo PROVAZNÍK, Edita JEKLOVÁ and Josef MAŠEK. Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater. \textit{Lékař a technika}. Praha: Czech Society for Biomedical Engineering and Medical Informatics, 2020, vol.~50, No~4, p.~137-141. ISSN~0301-5491. Available from: https://dx.doi.org/10.14311/CTJ.2020.4.03.

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