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@article{1808458,
author = {Malá, Miriam and Itterheimová, Petra and Homola, Lukáš and Vinohradská, Jana and Kubáň, Petr},
article_location = {Basel},
article_number = {12},
doi = {http://dx.doi.org/10.3390/separations8120234},
keywords = {cystic fibrosis; sweat sampling; skin-wash; capillary electrophoresis; contactless conductivity detection; ion ratio},
language = {eng},
issn = {2297-8739},
journal = {Separations},
title = {3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis},
url = {https://www.mdpi.com/2297-8739/8/12/234#cite},
volume = {8},
year = {2021}
}
TY - JOUR
ID - 1808458
AU - Malá, Miriam - Itterheimová, Petra - Homola, Lukáš - Vinohradská, Jana - Kubáň, Petr
PY - 2021
TI - 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis
JF - Separations
VL - 8
IS - 12
SP - 1-10
EP - 1-10
PB - MDPI
SN - 22978739
KW - cystic fibrosis
KW - sweat sampling
KW - skin-wash
KW - capillary electrophoresis
KW - contactless conductivity detection
KW - ion ratio
UR - https://www.mdpi.com/2297-8739/8/12/234#cite
N2 - Sweat chloride analysis is one of the important approaches in cystic fibrosis diagnosis. The commonly used Macroduct method to acquire sweat samples is semi-invasive, time consuming and expensive. Furthermore, this method often fails to collect a sufficient amount of sweat in newborns due to the insufficient sweating rate. In this work, we present a novel, simple, 3D-printed sampling device that is used to collect sweat specimens completely noninvasively in less than one minute. The device has a flow-through channel adjacent to the skin surface, through which 500 µL of deionized water is flushed and the spontaneously formed sweat on the skin in the channel area is washed into a plastic vial. The developed skin-wash procedure is a single step operation, is completely noninvasive and it always produces a sweat specimen. The ions from the skin-wash are subsequently analyzed by capillary electrophoresis with contactless conductivity detection and selected ion ratio (Cl−/K+) or ((Cl− + Na+)/K+) is used as a cut-off value to diagnose cystic fibrosis patients with sensitivity and specificity comparable to the conventional Macroduct method.
ER -
MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ and Petr KUBÁŇ. 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis. \textit{Separations}. Basel: MDPI, 2021, vol.~8, No~12, p.~1-10. ISSN~2297-8739. Available from: https://dx.doi.org/10.3390/separations8120234.
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