3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic
Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio
Analysis

J 2021

3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ, Petr KUBÁŇ et. al.

Basic information

Original name

3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis

Authors

MALÁ, Miriam (203 Czech Republic, belonging to the institution), Petra ITTERHEIMOVÁ (203 Czech Republic, belonging to the institution), Lukáš HOMOLA (203 Czech Republic, belonging to the institution), Jana VINOHRADSKÁ (203 Czech Republic, belonging to the institution) and Petr KUBÁŇ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Separations, Basel, MDPI, 2021, 2297-8739

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.344

RIV identification code

RIV/00216224:14110/21:00120173

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/separations8120234

UT WoS

000737166500001

Keywords in English

cystic fibrosis; sweat sampling; skin-wash; capillary electrophoresis; contactless conductivity detection; ion ratio

Abstract

V originále

Sweat chloride analysis is one of the important approaches in cystic fibrosis diagnosis. The commonly used Macroduct method to acquire sweat samples is semi-invasive, time consuming and expensive. Furthermore, this method often fails to collect a sufficient amount of sweat in newborns due to the insufficient sweating rate. In this work, we present a novel, simple, 3D-printed sampling device that is used to collect sweat specimens completely noninvasively in less than one minute. The device has a flow-through channel adjacent to the skin surface, through which 500 µL of deionized water is flushed and the spontaneously formed sweat on the skin in the channel area is washed into a plastic vial. The developed skin-wash procedure is a single step operation, is completely noninvasive and it always produces a sweat specimen. The ions from the skin-wash are subsequently analyzed by capillary electrophoresis with contactless conductivity detection and selected ion ratio (Cl−/K+) or ((Cl− + Na+)/K+) is used as a cut-off value to diagnose cystic fibrosis patients with sensitivity and specificity comparable to the conventional Macroduct method.

Links

NV18-08-00189, research and development project

Name: Nový postup diagnostiky cystické fibrózy založený na iontových poměrech v neinvazivně získaných vzorcích

Investor: Ministry of Health of the CR

Citovat

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ and Petr KUBÁŇ. 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis. Separations. Basel: MDPI, 2021, vol. 8, No 12, p. 1-10. ISSN 2297-8739. Available from: https://dx.doi.org/10.3390/separations8120234.

@article{1808458,
   author = {Malá, Miriam and Itterheimová, Petra and Homola, Lukáš and Vinohradská, Jana and Kubáň, Petr},
   article_location = {Basel},
   article_number = {12},
   doi = {http://dx.doi.org/10.3390/separations8120234},
   keywords = {cystic fibrosis; sweat sampling; skin-wash; capillary electrophoresis; contactless conductivity detection; ion ratio},
   language = {eng},
   issn = {2297-8739},
   journal = {Separations},
   title = {3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis},
   url = {https://www.mdpi.com/2297-8739/8/12/234#cite},
   volume = {8},
   year = {2021}
}

TY  - JOUR
ID  - 1808458
AU  - Malá, Miriam - Itterheimová, Petra - Homola, Lukáš - Vinohradská, Jana - Kubáň, Petr
PY  - 2021
TI  - 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis
JF  - Separations
VL  - 8
IS  - 12
SP  - 1-10
EP  - 1-10
PB  - MDPI
SN  - 22978739
KW  - cystic fibrosis
KW  - sweat sampling
KW  - skin-wash
KW  - capillary electrophoresis
KW  - contactless conductivity detection
KW  - ion ratio
UR  - https://www.mdpi.com/2297-8739/8/12/234#cite
N2  - Sweat chloride analysis is one of the important approaches in cystic fibrosis diagnosis. The commonly used Macroduct method to acquire sweat samples is semi-invasive, time consuming and expensive. Furthermore, this method often fails to collect a sufficient amount of sweat in newborns due to the insufficient sweating rate. In this work, we present a novel, simple, 3D-printed sampling device that is used to collect sweat specimens completely noninvasively in less than one minute. The device has a flow-through channel adjacent to the skin surface, through which 500 µL of deionized water is flushed and the spontaneously formed sweat on the skin in the channel area is washed into a plastic vial. The developed skin-wash procedure is a single step operation, is completely noninvasive and it always produces a sweat specimen. The ions from the skin-wash are subsequently analyzed by capillary electrophoresis with contactless conductivity detection and selected ion ratio (Cl−/K+) or ((Cl− + Na+)/K+) is used as a cut-off value to diagnose cystic fibrosis patients with sensitivity and specificity comparable to the conventional Macroduct method.
ER  -

MALÁ, Miriam, Petra ITTERHEIMOVÁ, Lukáš HOMOLA, Jana VINOHRADSKÁ and Petr KUBÁŇ. 3D Printed Skin-Wash Sampler for Sweat Sampling in Cystic Fibrosis Diagnosis Using Capillary Electrophoretic Ion Ratio Analysis. \textit{Separations}. Basel: MDPI, 2021, vol.~8, No~12, p.~1-10. ISSN~2297-8739. Available from: https://dx.doi.org/10.3390/separations8120234.

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