Pyocin-mediated antagonistic interactions in Pseudomonas spp.
isolated in James Ross Island, Antarctica

J 2022

Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ et. al.

Basic information

Original name

Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica

Authors

SNOPKOVÁ, Kateřina (203 Czech Republic, belonging to the institution), Kristýna DUFKOVÁ (203 Czech Republic, belonging to the institution), Ivo CHAMRÁD (203 Czech Republic), René LENOBEL (203 Czech Republic), Darina ČEJKOVÁ (203 Czech Republic), Marcel KOSINA (203 Czech Republic, belonging to the institution), Matěj HRALA (203 Czech Republic, belonging to the institution), Veronika HOLÁ (203 Czech Republic, belonging to the institution), Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

ENVIRONMENTAL MICROBIOLOGY, HOBOKEN, WILEY, 2022, 1462-2912

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.100

RIV identification code

RIV/00216224:14110/22:00125088

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1111/1462-2920.15809

UT WoS

000714374200001

Keywords in English

James Ross Island; Antarctica; Psychrotrophic Bacteria; P. aeruginosa; Microorganisms; Sequences; Plant; Phage; Pseudomonas; sp. nov.

Abstract

V originále

Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold-adapted microorganisms are still rare. Our study assessed the antimicrobial interactions of 36 Antarctic Pseudomonas spp. and described the genetic background of these interactions in selected strains. The overall bacteriocinogeny was greater compared to mesophilic Pseudomonas non-aeruginosa species. R-type tailocins were detected on transmission electron micrographs in 16 strains (44.4%); phylogenetic analysis of the corresponding gene clusters revealed that the P. prosekii CCM 8878 tailocin was related to the Rp3 group, whereas the tailocin in Pseudomonas sp. CCM 8880 to the Rp4 group. Soluble antimicrobials were produced by eight strains (22.-2%); gene mining found pyocin L homologues in the genomes of P. prosekii CCM 8881 and CCM 8879 and pyocin S9-like homologues in P. prosekii CCM 8881 and Pseudomonas sp. CCM 8880. Analysis of secretomes confirmed the production of all S- and L-type pyocin genes. Our results suggest that bacteriocin-based inhibition plays an important role in interactions among Antarctic soil bacteria, and these native, cold-adapted microorganisms could be a promising source of new antimicrobials.

Links

LM2015078, research and development project

Name: Česká polární výzkumná infrastruktura (Acronym: CzechPolar2)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015091, research and development project

Name: Národní centrum lékařské genomiky (Acronym: NCLG)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1325/2020, interní kód MU

Name: Biomedicínské vědy

Investor: Masaryk University

MUNI/A/1486/2020, interní kód MU

Name: Nové možnosti detekce a terapie nozokomiálních patogenů (Acronym: NMDTNP)

Investor: Masaryk University

Citovat

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ, Marcel KOSINA, Matěj HRALA, Veronika HOLÁ, Ivo SEDLÁČEK and David ŠMAJS. Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica. ENVIRONMENTAL MICROBIOLOGY. HOBOKEN: WILEY, 2022, vol. 24, No 3, p. 1294-1307. ISSN 1462-2912. Available from: https://dx.doi.org/10.1111/1462-2920.15809.

@article{1808897,
   author = {Snopková, Kateřina and Dufková, Kristýna and Chamrád, Ivo and Lenobel, René and Čejková, Darina and Kosina, Marcel and Hrala, Matěj and Holá, Veronika and Sedláček, Ivo and Šmajs, David},
   article_location = {HOBOKEN},
   article_number = {3},
   doi = {http://dx.doi.org/10.1111/1462-2920.15809},
   keywords = {James Ross Island; Antarctica; Psychrotrophic Bacteria; P. aeruginosa; Microorganisms; Sequences; Plant; Phage; Pseudomonas; sp. nov.},
   language = {eng},
   issn = {1462-2912},
   journal = {ENVIRONMENTAL MICROBIOLOGY},
   title = {Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica},
   url = {https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15809},
   volume = {24},
   year = {2022}
}

TY  - JOUR
ID  - 1808897
AU  - Snopková, Kateřina - Dufková, Kristýna - Chamrád, Ivo - Lenobel, René - Čejková, Darina - Kosina, Marcel - Hrala, Matěj - Holá, Veronika - Sedláček, Ivo - Šmajs, David
PY  - 2022
TI  - Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica
JF  - ENVIRONMENTAL MICROBIOLOGY
VL  - 24
IS  - 3
SP  - 1294-1307
EP  - 1294-1307
PB  - WILEY
SN  - 14622912
KW  - James Ross Island
KW  - Antarctica
KW  - Psychrotrophic Bacteria
KW  - P. aeruginosa
KW  - Microorganisms
KW  - Sequences
KW  - Plant
KW  - Phage
KW  - Pseudomonas
KW  - sp. nov.
UR  - https://sfamjournals.onlinelibrary.wiley.com/doi/10.1111/1462-2920.15809
N2  - Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold-adapted microorganisms are still rare. Our study assessed the antimicrobial interactions of 36 Antarctic Pseudomonas spp. and described the genetic background of these interactions in selected strains. The overall bacteriocinogeny was greater compared to mesophilic Pseudomonas non-aeruginosa species. R-type tailocins were detected on transmission electron micrographs in 16 strains (44.4%); phylogenetic analysis of the corresponding gene clusters revealed that the P. prosekii CCM 8878 tailocin was related to the Rp3 group, whereas the tailocin in Pseudomonas sp. CCM 8880 to the Rp4 group. Soluble antimicrobials were produced by eight strains (22.-2%); gene mining found pyocin L homologues in the genomes of P. prosekii CCM 8881 and CCM 8879 and pyocin S9-like homologues in P. prosekii CCM 8881 and Pseudomonas sp. CCM 8880. Analysis of secretomes confirmed the production of all S- and L-type pyocin genes. Our results suggest that bacteriocin-based inhibition plays an important role in interactions among Antarctic soil bacteria, and these native, cold-adapted microorganisms could be a promising source of new antimicrobials.
ER  -

SNOPKOVÁ, Kateřina, Kristýna DUFKOVÁ, Ivo CHAMRÁD, René LENOBEL, Darina ČEJKOVÁ, Marcel KOSINA, Matěj HRALA, Veronika HOLÁ, Ivo SEDLÁČEK and David ŠMAJS. Pyocin-mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica. \textit{ENVIRONMENTAL MICROBIOLOGY}. HOBOKEN: WILEY, 2022, vol.~24, No~3, p.~1294-1307. ISSN~1462-2912. Available from: https://dx.doi.org/10.1111/1462-2920.15809.

Detailed Information on Publication Record