J 2021

Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

RAŠKA, Jan, Hana KLÍMOVÁ, Kateřina SHEARDOVÁ, Veronika FEDOROVÁ, Hana HŘÍBKOVÁ et. al.

Basic information

Original name

Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

Authors

RAŠKA, Jan (203 Czech Republic, belonging to the institution), Hana KLÍMOVÁ (203 Czech Republic, belonging to the institution), Kateřina SHEARDOVÁ (203 Czech Republic), Veronika FEDOROVÁ (203 Czech Republic, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Veronika POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Simona VOCHYÁNOVÁ (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution) and Dáša BOHAČIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Stem cell research, Amsterdam, Elsevier, 2021, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.587

RIV identification code

RIV/00216224:14110/21:00120174

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2021.102378

UT WoS

000656247000002

Keywords in English

human iPSC lines; spontaneous late-onset Alzheimer’s disease

Tags

14110517, rivok

Tags

International impact, Reviewed
Změněno: 13/12/2021 12:17, Mgr. Tereza Miškechová

Abstract

V originále

Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.

Links

MUNI/R/1697/2020, interní kód MU
Name: Career Restart - Stem Cell-Based Models of Neurodegeneration
Investor: Masaryk University, CAREER RESTART
NV19-08-00472, research and development project
Name: Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí (Acronym: BioMAD)
Investor: Ministry of Health of the CR
ROZV/23/LF19/2019, interní kód MU
Name: Využití in vitro kultivovaných cerebrálních organoidů pro modelování patogeneze Alzheimerovy choroby
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
ROZV/28/LF22/2020, interní kód MU
Name: ln vitro modelováni Alzheimerovy choroby pomoci nově derivovaných indukovaných plunpotentnich kmenových buněk z pacientu se spontánní formou onemocnění.
Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects
Displayed: 9/11/2024 22:45