Generation of three human iPSC lines from patients with a
spontaneous late-onset Alzheimer's disease and three sex- and
age-matched healthy controls

J 2021

Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

RAŠKA, Jan, Hana KLÍMOVÁ, Kateřina SHEARDOVÁ, Veronika FEDOROVÁ, Hana HŘÍBKOVÁ et. al.

Basic information

Original name

Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls

Authors

RAŠKA, Jan (203 Czech Republic, belonging to the institution), Hana KLÍMOVÁ (203 Czech Republic, belonging to the institution), Kateřina SHEARDOVÁ (203 Czech Republic), Veronika FEDOROVÁ (203 Czech Republic, belonging to the institution), Hana HŘÍBKOVÁ (203 Czech Republic, belonging to the institution), Veronika POSPÍŠILOVÁ (203 Czech Republic, belonging to the institution), Simona VOCHYÁNOVÁ (203 Czech Republic, belonging to the institution), Tereza VÁŇOVÁ (203 Czech Republic, belonging to the institution) and Dáša BOHAČIAKOVÁ (703 Slovakia, guarantor, belonging to the institution)

Edition

Stem cell research, Amsterdam, Elsevier, 2021, 1873-5061

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10601 Cell biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.587

RIV identification code

RIV/00216224:14110/21:00120174

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.scr.2021.102378

UT WoS

000656247000002

Keywords in English

human iPSC lines; spontaneous late-onset Alzheimer’s disease

Abstract

V originále

Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.

Links

MUNI/R/1697/2020, interní kód MU

Name: Career Restart - Stem Cell-Based Models of Neurodegeneration

Investor: Masaryk University, CAREER RESTART

NV19-08-00472, research and development project

Name: Klinicky relevantní biochemické, imunologické a buněčné biomarkery Alzheimerovy nemoci a stárnutí (Acronym: BioMAD)

Investor: Ministry of Health of the CR

ROZV/23/LF19/2019, interní kód MU

Name: Využití in vitro kultivovaných cerebrálních organoidů pro modelování patogeneze Alzheimerovy choroby

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

ROZV/28/LF22/2020, interní kód MU

Name: ln vitro modelováni Alzheimerovy choroby pomoci nově derivovaných indukovaných plunpotentnich kmenových buněk z pacientu se spontánní formou onemocnění.

Investor: Ministry of Education, Youth and Sports of the CR, Internal development projects

Citovat

RAŠKA, Jan, Hana KLÍMOVÁ, Kateřina SHEARDOVÁ, Veronika FEDOROVÁ, Hana HŘÍBKOVÁ, Veronika POSPÍŠILOVÁ, Simona VOCHYÁNOVÁ, Tereza VÁŇOVÁ and Dáša BOHAČIAKOVÁ. Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls. Stem cell research. Amsterdam: Elsevier, 2021, vol. 53, May 2021, p. 1-5. ISSN 1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2021.102378.

@article{1809519,
   author = {Raška, Jan and Klímová, Hana and Sheardová, Kateřina and Fedorová, Veronika and Hříbková, Hana and Pospíšilová, Veronika and Vochyánová, Simona and Váňová, Tereza and Bohačiaková, Dáša},
   article_location = {Amsterdam},
   article_number = {May 2021},
   doi = {http://dx.doi.org/10.1016/j.scr.2021.102378},
   keywords = {human iPSC lines; spontaneous late-onset Alzheimer’s disease},
   language = {eng},
   issn = {1873-5061},
   journal = {Stem cell research},
   title = {Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls},
   url = {https://www.sciencedirect.com/science/article/pii/S1873506121002245?via%3Dihub},
   volume = {53},
   year = {2021}
}

TY  - JOUR
ID  - 1809519
AU  - Raška, Jan - Klímová, Hana - Sheardová, Kateřina - Fedorová, Veronika - Hříbková, Hana - Pospíšilová, Veronika - Vochyánová, Simona - Váňová, Tereza - Bohačiaková, Dáša
PY  - 2021
TI  - Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls
JF  - Stem cell research
VL  - 53
IS  - May 2021
SP  - 1-5
EP  - 1-5
PB  - Elsevier
SN  - 18735061
KW  - human iPSC lines
KW  - spontaneous late-onset Alzheimer’s disease
UR  - https://www.sciencedirect.com/science/article/pii/S1873506121002245?via%3Dihub
N2  - Human induced pluripotent stem cell (iPSC) lines were generated from patients with spontaneous late-onset Alzheimer's disease (AD) and three healthy control individuals. Peripheral blood mononuclear cells were reprogrammed with Yamanaka factors (OSKM) using a commercially available Epi5 Reprogramming Kit. The pluripotency of iPSCs was confirmed by the expression of pluripotency factors and by their ability to differentiate to all three germ layers in vitro. Newly derived cell lines can be used to model Alzheimer's disease in vitro.
ER  -

RAŠKA, Jan, Hana KLÍMOVÁ, Kateřina SHEARDOVÁ, Veronika FEDOROVÁ, Hana HŘÍBKOVÁ, Veronika POSPÍŠILOVÁ, Simona VOCHYÁNOVÁ, Tereza VÁŇOVÁ and Dáša BOHAČIAKOVÁ. Generation of three human iPSC lines from patients with a spontaneous late-onset Alzheimer's disease and three sex- and age-matched healthy controls. \textit{Stem cell research}. Amsterdam: Elsevier, 2021, vol.~53, May 2021, p.~1-5. ISSN~1873-5061. Available from: https://dx.doi.org/10.1016/j.scr.2021.102378.

Detailed Information on Publication Record