De novo developed microsatellite markers in gill parasites of
the genus Dactylogyrus (Monogenea): Revealing the
phylogeographic pattern of population structure in the
generalist parasite Dactylogyrus vistulae

J 2021

De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae

BENOVICS, Michal, Lenka GETTOVÁ and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ

Basic information

Original name

De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae

Authors

BENOVICS, Michal (703 Slovakia, guarantor, belonging to the institution), Lenka GETTOVÁ (703 Slovakia, belonging to the institution) and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Ecology and Evolution, Hoboken, Wiley, 2021, 2045-7758

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.167

RIV identification code

RIV/00216224:14310/21:00119426

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1002/ece3.8230

UT WoS

000716862600001

Keywords in English

Cyprinoidei; historical dispersion; host-specific parasites; polymorphic markers; population genetics

Abstract

V originále

Approaches using microsatellite markers are considered the gold standard for modern population genetic studies. However, although they have found application in research into various platyhelminth taxa, they remained substantially underutilized in the study of monogeneans. In the present study, a newly developed set of 24 microsatellite markers was used to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans (mean N-A per locus = 3.917), suggesting that generalist D. vistulae expanded from the south to the north in the Balkans and later into central Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, the partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier for gene flow, even for generalist parasite species.

Links

GA20-13539S, research and development project

Name: Paraziti odhalují historické a součastné kontakty kaprovitých hostitelů: role Blízkého východu v biogeografii západního Palearktu

Investor: Czech Science Foundation

Citovat

BENOVICS, Michal, Lenka GETTOVÁ and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae. Ecology and Evolution. Hoboken: Wiley, 2021, vol. 11, No 23, p. 16585-16599. ISSN 2045-7758. Available from: https://dx.doi.org/10.1002/ece3.8230.

@article{1810457,
   author = {Benovics, Michal and Gettová, Lenka and Vetešníková Šimková, Andrea},
   article_location = {Hoboken},
   article_number = {23},
   doi = {http://dx.doi.org/10.1002/ece3.8230},
   keywords = {Cyprinoidei; historical dispersion; host-specific parasites; polymorphic markers; population genetics},
   language = {eng},
   issn = {2045-7758},
   journal = {Ecology and Evolution},
   title = {De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae},
   url = {https://doi.org/10.1002/ece3.8230},
   volume = {11},
   year = {2021}
}

TY  - JOUR
ID  - 1810457
AU  - Benovics, Michal - Gettová, Lenka - Vetešníková Šimková, Andrea
PY  - 2021
TI  - De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae
JF  - Ecology and Evolution
VL  - 11
IS  - 23
SP  - 16585-16599
EP  - 16585-16599
PB  - Wiley
SN  - 20457758
KW  - Cyprinoidei
KW  - historical dispersion
KW  - host-specific parasites
KW  - polymorphic markers
KW  - population genetics
UR  - https://doi.org/10.1002/ece3.8230
N2  - Approaches using microsatellite markers are considered the gold standard for modern population genetic studies. However, although they have found application in research into various platyhelminth taxa, they remained substantially underutilized in the study of monogeneans. In the present study, a newly developed set of 24 microsatellite markers was used to investigate the genetic diversity of the generalist monogenean species Dactylogyrus vistulae. The analyzed parasite specimens were collected from 13 cyprinoid species from 11 sites in the Apennine and Balkan peninsulas. A total of 159 specimens were genotyped at each of the loci and the number of alleles per locus ranged from 2 to 16, with a mean number of 6.958 alleles per locus. Exceptionally high genetic diversity was observed among D. vistulae individuals in the southern Balkans (mean N-A per locus = 3.917), suggesting that generalist D. vistulae expanded from the south to the north in the Balkans and later into central Europe. The initial clustering analysis divided all investigated specimens into three major clusters; however, the results of the subsequent analyses revealed the existence of various subpopulations, suggesting that the population structure of D. vistulae is associated with the diversification of their cyprinoid hosts. In addition, the partition of the parasite population was observed in regions of the sympatric occurrence of two host species, indicating that these hosts may represent a barrier for gene flow, even for generalist parasite species.
ER  -

BENOVICS, Michal, Lenka GETTOVÁ and Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De novo developed microsatellite markers in gill parasites of the genus Dactylogyrus (Monogenea): Revealing the phylogeographic pattern of population structure in the generalist parasite Dactylogyrus vistulae. \textit{Ecology and Evolution}. Hoboken: Wiley, 2021, vol.~11, No~23, p.~16585-16599. ISSN~2045-7758. Available from: https://dx.doi.org/10.1002/ece3.8230.

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