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@article{1811684, author = {Śliwińska, Elwira and Loureiro, Joao and Leitch, Ilia J. and Šmarda, Petr and Bainard, Jillian and Bureš, Petr and Chumová, Zuzana and Horová, Lucie and Koutecký, Petr and Lučanová, Magdaléna and Travníček, Pavel and Galbraith, David W.}, article_location = {Hoboken}, article_number = {9}, doi = {http://dx.doi.org/10.1002/cyto.a.24499}, keywords = {cell cycle; DNA base composition; DNA content; endoreduplication; flow cytometric seed screening; genome size; in vitro cultures; intraspecific variation; ploidy}, language = {eng}, issn = {1552-4922}, journal = {Cytometry: Part A}, title = {Application-based guidelines for best practices in plant flow cytometry}, url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499}, volume = {101}, year = {2022} }
TY - JOUR ID - 1811684 AU - Śliwińska, Elwira - Loureiro, Joao - Leitch, Ilia J. - Šmarda, Petr - Bainard, Jillian - Bureš, Petr - Chumová, Zuzana - Horová, Lucie - Koutecký, Petr - Lučanová, Magdaléna - Travníček, Pavel - Galbraith, David W. PY - 2022 TI - Application-based guidelines for best practices in plant flow cytometry JF - Cytometry: Part A VL - 101 IS - 9 SP - 749-781 EP - 749-781 PB - Wiley SN - 15524922 KW - cell cycle KW - DNA base composition KW - DNA content KW - endoreduplication KW - flow cytometric seed screening KW - genome size KW - in vitro cultures KW - intraspecific variation KW - ploidy UR - https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.a.24499 N2 - Flow cytometry (FCM) is currently the most widely-used method to establish nuclearDNA content in plants. Since simple, 1-3-parameter, flow cytometers, which are suffi-cient for most plant applications, are commercially available at a reasonable price, thenumber of laboratories equipped with these instruments, and consequently newFCM users, has greatly increased over the last decade. This paper meets an urgentneed for comprehensive recommendations for best practices in FCM for differentplant science applications. We discuss advantages and limitations of establishing plantploidy, genome size, DNA base composition, cell cycle activity, and level of endo-reduplication. Applications of such measurements in plant systematics, ecology,molecular biology research, reproduction biology, tissue cultures, plant breeding, andseed sciences are described. Advice is included on how to obtain accurate and reliableresults, as well as how to manage troubleshooting that may occur during samplepreparation, cytometric measurements, and data handling. Each section is followedby best practice recommendations; tips as to what specific information should beprovided in FCM papers are also provide ER -
$\backslash$'SLIWI$\backslash$'NSKA, Elwira, Joao LOUREIRO, Ilia J. LEITCH, Petr ŠMARDA, Jillian BAINARD, Petr BUREŠ, Zuzana CHUMOVÁ, Lucie HOROVÁ, Petr KOUTECKÝ, Magdaléna LUČANOVÁ, Pavel TRAVNÍČEK and David W. GALBRAITH. Application-based guidelines for best practices in plant flow cytometry. \textit{Cytometry: Part A}. Hoboken: Wiley, 2022, vol.~101, No~9, p.~749-781. ISSN~1552-4922. Available from: https://dx.doi.org/10.1002/cyto.a.24499.
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