RPS15 mutations rewire RNA translation in chronic lymphocytic
leukemia

J 2021

RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia

NTOUFA, S., M. GEROUSI, S. LAIDOU, F. PSOMOPOULOS, G. TSIOLAS et. al.

Basic information

Original name

RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia

Authors

NTOUFA, S., M. GEROUSI, S. LAIDOU, F. PSOMOPOULOS, G. TSIOLAS, T. MOYSIADIS, N. PAPAKONSTANTINOU, L. MANSOURI, A. ANAGNOSTOPOULOS, N. STAVROGIANNI, Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution), Karla PLEVOVÁ (203 Czech Republic, belonging to the institution), A.M. MAKRIS, R. ROSENQUIST and K. STAMATOPOULOS

Edition

BLOOD ADVANCES, WASHINGTON, AMER SOC HEMATOLOGY, 2021, 2473-9529

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30205 Hematology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.637

RIV identification code

RIV/00216224:14740/21:00120184

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1182/bloodadvances.2020001717

UT WoS

000672759900014

Keywords in English

EXPRESSION; IMPACT; CLL

Abstract

V originále

Recent studies of chronic lymphocytic leukemia (CLL) have reported recurrent mutations in the RPS15 gene, which encodes the ribosomal protein S15 (RPS15), a component of the 40S ribosomal subunit. Despite some evidence about the role of mutant RPS15 (mostly obtained from the analysis of cell lines), the precise impact of RPS15 mutations on the translational program in primary CLL cells remains largely unexplored. Here, using RNA sequencing and ribosome profiling, a technique that involves measuring translational efficiency, we sought to obtain global insight into changes in translation induced by RPS15 mutations in CLL cells. To this end, we evaluated primary CLL cells from patients with wildtype or mutant RPS15 as well as MEC1 CLL cells transfected with mutant or wild-type RPS15. Our data indicate that RPS15 mutations rewire the translation program of primary CLL cells by reducing their translational efficiency, an effect not seen in MEC1 cells. In detail, RPS15 mutant primary CLL cells displayed altered translation efficiency of other ribosomal proteins and regulatory elements that affect key cell processes, such as the translational machinery and immune signaling, as well as genes known to be implicated in CLL, hence highlighting a relevant role for RPS15 in the natural history of CLL.

Links

NU21-08-00237, research and development project

Name: Pokročilé sekvenační metody pro analýzu strukturních přestaveb nádorového genomu

Investor: Ministry of Health of the CR, Advanced sequencing methods for deciphering structural variants in cancer genome, Subprogram 1 - standard

NV19-03-00091, research and development project

Name: Komplexní prognostický a prediktivní panel pro pacienty s chronickou lymfocytární leukémií: nástroj sekvenování nové generace vhodný pro klinickou praxi i studium genetického pozadí průběhu choroby

Investor: Ministry of Health of the CR

Citovat

NTOUFA, S., M. GEROUSI, S. LAIDOU, F. PSOMOPOULOS, G. TSIOLAS, T. MOYSIADIS, N. PAPAKONSTANTINOU, L. MANSOURI, A. ANAGNOSTOPOULOS, N. STAVROGIANNI, Šárka POSPÍŠILOVÁ, Karla PLEVOVÁ, A.M. MAKRIS, R. ROSENQUIST and K. STAMATOPOULOS. RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia. BLOOD ADVANCES. WASHINGTON: AMER SOC HEMATOLOGY, 2021, vol. 5, No 13, p. 2788-2792. ISSN 2473-9529. Available from: https://dx.doi.org/10.1182/bloodadvances.2020001717.

@article{1813386,
   author = {Ntoufa, S. and Gerousi, M. and Laidou, S. and Psomopoulos, F. and Tsiolas, G. and Moysiadis, T. and Papakonstantinou, N. and Mansouri, L. and Anagnostopoulos, A. and Stavrogianni, N. and Pospíšilová, Šárka and Plevová, Karla and Makris, A.M. and Rosenquist, R. and Stamatopoulos, K.},
   article_location = {WASHINGTON},
   article_number = {13},
   doi = {http://dx.doi.org/10.1182/bloodadvances.2020001717},
   keywords = {EXPRESSION; IMPACT; CLL},
   language = {eng},
   issn = {2473-9529},
   journal = {BLOOD ADVANCES},
   title = {RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia},
   url = {https://ashpublications.org/bloodadvances/article/5/13/2788/476283/RPS15-mutations-rewire-RNA-translation-in-chronic},
   volume = {5},
   year = {2021}
}

TY  - JOUR
ID  - 1813386
AU  - Ntoufa, S. - Gerousi, M. - Laidou, S. - Psomopoulos, F. - Tsiolas, G. - Moysiadis, T. - Papakonstantinou, N. - Mansouri, L. - Anagnostopoulos, A. - Stavrogianni, N. - Pospíšilová, Šárka - Plevová, Karla - Makris, A.M. - Rosenquist, R. - Stamatopoulos, K.
PY  - 2021
TI  - RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia
JF  - BLOOD ADVANCES
VL  - 5
IS  - 13
SP  - 2788-2792
EP  - 2788-2792
PB  - AMER SOC HEMATOLOGY
SN  - 24739529
KW  - EXPRESSION
KW  - IMPACT
KW  - CLL
UR  - https://ashpublications.org/bloodadvances/article/5/13/2788/476283/RPS15-mutations-rewire-RNA-translation-in-chronic
N2  - Recent studies of chronic lymphocytic leukemia (CLL) have reported recurrent mutations in the RPS15 gene, which encodes the ribosomal protein S15 (RPS15), a component of the 40S ribosomal subunit. Despite some evidence about the role of mutant RPS15 (mostly obtained from the analysis of cell lines), the precise impact of RPS15 mutations on the translational program in primary CLL cells remains largely unexplored. Here, using RNA sequencing and ribosome profiling, a technique that involves measuring translational efficiency, we sought to obtain global insight into changes in translation induced by RPS15 mutations in CLL cells. To this end, we evaluated primary CLL cells from patients with wildtype or mutant RPS15 as well as MEC1 CLL cells transfected with mutant or wild-type RPS15. Our data indicate that RPS15 mutations rewire the translation program of primary CLL cells by reducing their translational efficiency, an effect not seen in MEC1 cells. In detail, RPS15 mutant primary CLL cells displayed altered translation efficiency of other ribosomal proteins and regulatory elements that affect key cell processes, such as the translational machinery and immune signaling, as well as genes known to be implicated in CLL, hence highlighting a relevant role for RPS15 in the natural history of CLL.
ER  -

NTOUFA, S., M. GEROUSI, S. LAIDOU, F. PSOMOPOULOS, G. TSIOLAS, T. MOYSIADIS, N. PAPAKONSTANTINOU, L. MANSOURI, A. ANAGNOSTOPOULOS, N. STAVROGIANNI, Šárka POSPÍŠILOVÁ, Karla PLEVOVÁ, A.M. MAKRIS, R. ROSENQUIST and K. STAMATOPOULOS. RPS15 mutations rewire RNA translation in chronic lymphocytic leukemia. \textit{BLOOD ADVANCES}. WASHINGTON: AMER SOC HEMATOLOGY, 2021, vol.~5, No~13, p.~2788-2792. ISSN~2473-9529. Available from: https://dx.doi.org/10.1182/bloodadvances.2020001717.

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