RGDS-Modified Superporous Poly(2-Hydroxyethyl
Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

J 2021

RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN et. al.

Basic information

Original name

RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model

Authors

SVOZILOVÁ, Hana (203 Czech Republic, belonging to the institution), Z. PLICHTA, V. PROKS, Radana STUDENÁ (203 Czech Republic, belonging to the institution), Jiří BALOUN (203 Czech Republic, belonging to the institution), Michael DOUBEK (203 Czech Republic, belonging to the institution), Šárka POSPÍŠILOVÁ (203 Czech Republic, guarantor, belonging to the institution) and D. HORAK

Edition

International Journal of Molecular Sciences, Basel, Multidisciplinary Digital Publishing Institute, 2021, 1422-0067

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 6.208

RIV identification code

RIV/00216224:14740/21:00123378

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms22052376

UT WoS

000628297600001

Keywords in English

poly(2-hydroxyethyl methacrylate); 3D scaffold; RGDS; chronic lymphocytic leukemia; B cell survival

Abstract

V originále

Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals in the polymerization mixture. To improve cell vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide is immobilized on the N-(gamma-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction of SH with maleimide groups. This modification is especially suitable for the survival of primary chronic lymphocytic leukemia cells (B-CLLs) in 3D cell culture. No other tested stimuli (interleukin-4, CD40 ligand, or shaking) can further improve B-CLL survival or metabolic activity. Both unmodified and RGDS-modified P(HEMA-AEMA) scaffolds serve as a long-term (70 days) 3D culture platforms for HS-5 and M2-10B4 bone marrow stromal cell lines and MEC-1 and HG-3 B-CLL cell lines, although the adherent cells retain their physiological morphologies, preferably on RGDS-modified hydrogels. Moreover, the porosity of hydrogels allows direct cell lysis, followed by efficient DNA isolation from the 3D-cultured cells. P(HEMA-AEMA)-RGDS thus serves as a suitable 3D in vitro leukemia model that enables molecular and metabolic assays and allows imaging of cell morphology, interactions, and migration by confocal microscopy. Such applications can prospectively assist in testing of drugs to treat this frequently recurring or refractory cancer.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1595/2020, interní kód MU

Name: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit VIII (Acronym: VýDiTeHeMa VIII)

Investor: Masaryk University

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN, Michael DOUBEK, Šárka POSPÍŠILOVÁ and D. HORAK. RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model. International Journal of Molecular Sciences. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol. 22, No 5, p. 2376-2392. ISSN 1422-0067. Available from: https://dx.doi.org/10.3390/ijms22052376.

@article{1813387,
   author = {Svozilová, Hana and Plichta, Z. and Proks, V. and Studená, Radana and Baloun, Jiří and Doubek, Michael and Pospíšilová, Šárka and Horak, D.},
   article_location = {Basel},
   article_number = {5},
   doi = {http://dx.doi.org/10.3390/ijms22052376},
   keywords = {poly(2-hydroxyethyl methacrylate); 3D scaffold; RGDS; chronic lymphocytic leukemia; B cell survival},
   language = {eng},
   issn = {1422-0067},
   journal = {International Journal of Molecular Sciences},
   title = {RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model},
   url = {https://www.mdpi.com/1422-0067/22/5/2376},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1813387
AU  - Svozilová, Hana - Plichta, Z. - Proks, V. - Studená, Radana - Baloun, Jiří - Doubek, Michael - Pospíšilová, Šárka - Horak, D.
PY  - 2021
TI  - RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 5
SP  - 2376
EP  - 2376
PB  - Multidisciplinary Digital Publishing Institute
SN  - 14220067
KW  - poly(2-hydroxyethyl methacrylate)
KW  - 3D scaffold
KW  - RGDS
KW  - chronic lymphocytic leukemia
KW  - B cell survival
UR  - https://www.mdpi.com/1422-0067/22/5/2376
N2  - Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals in the polymerization mixture. To improve cell vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide is immobilized on the N-(gamma-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction of SH with maleimide groups. This modification is especially suitable for the survival of primary chronic lymphocytic leukemia cells (B-CLLs) in 3D cell culture. No other tested stimuli (interleukin-4, CD40 ligand, or shaking) can further improve B-CLL survival or metabolic activity. Both unmodified and RGDS-modified P(HEMA-AEMA) scaffolds serve as a long-term (70 days) 3D culture platforms for HS-5 and M2-10B4 bone marrow stromal cell lines and MEC-1 and HG-3 B-CLL cell lines, although the adherent cells retain their physiological morphologies, preferably on RGDS-modified hydrogels. Moreover, the porosity of hydrogels allows direct cell lysis, followed by efficient DNA isolation from the 3D-cultured cells. P(HEMA-AEMA)-RGDS thus serves as a suitable 3D in vitro leukemia model that enables molecular and metabolic assays and allows imaging of cell morphology, interactions, and migration by confocal microscopy. Such applications can prospectively assist in testing of drugs to treat this frequently recurring or refractory cancer.
ER  -

SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN, Michael DOUBEK, Šárka POSPÍŠILOVÁ and D. HORAK. RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol.~22, No~5, p.~2376-2392. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22052376.

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