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@article{1813387,
author = {Svozilová, Hana and Plichta, Z. and Proks, V. and Studená, Radana and Baloun, Jiří and Doubek, Michael and Pospíšilová, Šárka and Horak, D.},
article_location = {Basel},
article_number = {5},
doi = {http://dx.doi.org/10.3390/ijms22052376},
keywords = {poly(2-hydroxyethyl methacrylate); 3D scaffold; RGDS; chronic lymphocytic leukemia; B cell survival},
language = {eng},
issn = {1422-0067},
journal = {International Journal of Molecular Sciences},
title = {RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model},
url = {https://www.mdpi.com/1422-0067/22/5/2376},
volume = {22},
year = {2021}
}
TY - JOUR
ID - 1813387
AU - Svozilová, Hana - Plichta, Z. - Proks, V. - Studená, Radana - Baloun, Jiří - Doubek, Michael - Pospíšilová, Šárka - Horak, D.
PY - 2021
TI - RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model
JF - International Journal of Molecular Sciences
VL - 22
IS - 5
SP - 2376
EP - 2376
PB - Multidisciplinary Digital Publishing Institute
SN - 14220067
KW - poly(2-hydroxyethyl methacrylate)
KW - 3D scaffold
KW - RGDS
KW - chronic lymphocytic leukemia
KW - B cell survival
UR - https://www.mdpi.com/1422-0067/22/5/2376
N2 - Superporous poly(2-hydroxyethyl methacrylate-co-2-aminoethyl methacrylate) (P(HEMA-AEMA)) hydrogel scaffolds are designed for in vitro 3D culturing of leukemic B cells. Hydrogel porosity, which influences cell functions and growth, is introduced by adding ammonium oxalate needle-like crystals in the polymerization mixture. To improve cell vitality, cell-adhesive Arg-Gly-Asp-Ser (RGDS) peptide is immobilized on the N-(gamma-maleimidobutyryloxy)succinimide-activated P(HEMA-AEMA) hydrogels via reaction of SH with maleimide groups. This modification is especially suitable for the survival of primary chronic lymphocytic leukemia cells (B-CLLs) in 3D cell culture. No other tested stimuli (interleukin-4, CD40 ligand, or shaking) can further improve B-CLL survival or metabolic activity. Both unmodified and RGDS-modified P(HEMA-AEMA) scaffolds serve as a long-term (70 days) 3D culture platforms for HS-5 and M2-10B4 bone marrow stromal cell lines and MEC-1 and HG-3 B-CLL cell lines, although the adherent cells retain their physiological morphologies, preferably on RGDS-modified hydrogels. Moreover, the porosity of hydrogels allows direct cell lysis, followed by efficient DNA isolation from the 3D-cultured cells. P(HEMA-AEMA)-RGDS thus serves as a suitable 3D in vitro leukemia model that enables molecular and metabolic assays and allows imaging of cell morphology, interactions, and migration by confocal microscopy. Such applications can prospectively assist in testing of drugs to treat this frequently recurring or refractory cancer.
ER -
SVOZILOVÁ, Hana, Z. PLICHTA, V. PROKS, Radana STUDENÁ, Jiří BALOUN, Michael DOUBEK, Šárka POSPÍŠILOVÁ and D. HORAK. RGDS-Modified Superporous Poly(2-Hydroxyethyl Methacrylate)-Based Scaffolds as 3D In Vitro Leukemia Model. \textit{International Journal of Molecular Sciences}. Basel: Multidisciplinary Digital Publishing Institute, 2021, vol.~22, No~5, p.~2376-2392. ISSN~1422-0067. Available from: https://dx.doi.org/10.3390/ijms22052376.
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