Increased TRPV1 gene expression in the tissues of Barrett's
esophagus and esophageal adenocarcinoma

a 2021

Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zdeněk KALA, Lumír KUNOVSKÝ, Jiří DOLINA et. al.

Basic information

Original name

Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma

Authors

DEISSOVÁ, Tereza (203 Czech Republic), Lydie IZAKOVIČOVÁ HOLLÁ (203 Czech Republic), Zdeněk KALA (203 Czech Republic), Lumír KUNOVSKÝ (203 Czech Republic), Jiří DOLINA (203 Czech Republic), Ondřej SLABÝ (203 Czech Republic) and Petra BOŘILOVÁ LINHARTOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

GASTRO 2021 Prague, 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

30101 Human genetics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14110/21:00120191

Organization unit

Faculty of Medicine

Keywords in English

gastroesophageal reflux disease; TRPV1; gene expression; single nucleotide polymorphisms

Změněno: 15/3/2022 21:07, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Abstract

V originále

Aim: Gastroesophageal reflux disease (GERD) occurs when the stomach contents return back to the esophagus and causes symptoms and/or complications such as reflux esophagitis (RE), Barrett's esophagus (BE) or esophageal adenocarcinoma (EAC). The transient receptor potential vanilloid subtype 1 (TRPV1) is main acid receptor, whose up-regulation plays an important role in the development of distal esophageal inflammation and reflux symptoms. The aim of this study was to analyze two functional single nucleotide polymorphisms (SNPs) in TRPV1 gene and its expression in esophageal tissue in patients with GERD. Methods: Two SNPs (C/G rs222747; C/T rs8065080) in TRPV1 gene were determined in the 325 patients with GERD (142 with RE; 61 with BE; 22 with EAC) and 101 healthy controls. The TRPV1 mRNA expression was analyzed in pathological and healthy esophageal tissues of 10 patients with RE, 8 with BE and 7 with EAC. Both types of experiments were based on TaqMan® polymerase chain reaction method. For statistical evaluation was used the Statistica v13.2 software. Results: The allele, genotype, haplogenotype or haplotype frequencies of study SNPs between patients with GERD or RE/BE/EAC separately and healthy controls were similar (p>0.05). The higher mRNA TRPV1 expression was found in the tissues of BE (p=0.017) and EAC (p=0.018) compared to healthy tissues. Conclusion: There was found increased mRNA TRPV1 expression in the pathological esophageal tissues of patients with BE or EAC who might benefit with pharmacological modulation of TRPV1 receptor.

Links

NU20-03-00126, research and development project

Name: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministry of Health of the CR, Host microbiome in relation to Barrett ́s esophagus and esophageal adenocarcinoma development, Subprogram 1 - standard

Citovat

DEISSOVÁ, Tereza, Lydie IZAKOVIČOVÁ HOLLÁ, Zdeněk KALA, Lumír KUNOVSKÝ, Jiří DOLINA, Ondřej SLABÝ and Petra BOŘILOVÁ LINHARTOVÁ. Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma. In GASTRO 2021 Prague. 2021.

@proceedings{1814658,
   author = {Deissová, Tereza and Izakovičová Hollá, Lydie and Kala, Zdeněk and Kunovský, Lumír and Dolina, Jiří and Slabý, Ondřej and Bořilová Linhartová, Petra},
   booktitle = {GASTRO 2021 Prague},
   keywords = {gastroesophageal reflux disease; TRPV1; gene expression; single nucleotide polymorphisms},
   language = {eng},
   title = {Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma},
   year = {2021}
}

TY  - CONF
ID  - 1814658
AU  - Deissová, Tereza - Izakovičová Hollá, Lydie - Kala, Zdeněk - Kunovský, Lumír - Dolina, Jiří - Slabý, Ondřej - Bořilová Linhartová, Petra
PY  - 2021
TI  - Increased TRPV1 gene expression in the tissues of Barrett's esophagus and esophageal adenocarcinoma
KW  - gastroesophageal reflux disease
KW  - TRPV1
KW  - gene expression
KW  - single nucleotide polymorphisms
N2  - Aim: Gastroesophageal reflux disease (GERD) occurs when the stomach contents return back to the esophagus and causes symptoms and/or complications such as reflux esophagitis (RE), Barrett's esophagus (BE) or esophageal adenocarcinoma (EAC). The transient receptor potential vanilloid subtype 1 (TRPV1) is main acid receptor, whose up-regulation plays an important role in the development of distal esophageal inflammation and reflux symptoms. The aim of this study was to analyze two functional single nucleotide polymorphisms (SNPs) in TRPV1 gene and its expression in esophageal tissue in patients with GERD. Methods: Two SNPs (C/G rs222747; C/T rs8065080) in TRPV1 gene were determined in the 325 patients with GERD (142 with RE; 61 with BE; 22 with EAC) and 101 healthy controls. The TRPV1 mRNA expression was analyzed in pathological and healthy esophageal tissues of 10 patients with RE, 8 with BE and 7 with EAC. Both types of experiments were based on TaqMan® polymerase chain reaction method. For statistical evaluation was used the Statistica v13.2 software. Results: The allele, genotype, haplogenotype or haplotype frequencies of study SNPs between patients with GERD or RE/BE/EAC separately and healthy controls were similar (p>0.05). The higher mRNA TRPV1 expression was found in the tissues of BE (p=0.017) and EAC (p=0.018) compared to healthy tissues. Conclusion: There was found increased mRNA TRPV1 expression in the pathological esophageal tissues of patients with BE or EAC who might benefit with pharmacological modulation of TRPV1 receptor.
ER  -

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