Cargo Release from Nonenveloped Viruses and Virus-like
Nanoparticles: Capsid Rupture or Pore Formation

J 2021

Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation

SUKENÍK, Lukáš, Liya MUKHAMEDOVA, Michaela PROCHÁZKOVÁ, Karel ŠKUBNÍK, Pavel PLEVKA et. al.

Basic information

Original name

Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation

Authors

SUKENÍK, Lukáš (203 Czech Republic, belonging to the institution), Liya MUKHAMEDOVA (643 Russian Federation, belonging to the institution), Michaela PROCHÁZKOVÁ (203 Czech Republic, belonging to the institution), Karel ŠKUBNÍK (203 Czech Republic, belonging to the institution), Pavel PLEVKA (203 Czech Republic, belonging to the institution) and Robert VÁCHA (203 Czech Republic, guarantor, belonging to the institution)

Edition

ACS Nano, Washington, D.C. American Chemical Society, 2021, 1936-0851

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10607 Virology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 18.027

RIV identification code

RIV/00216224:14740/21:00119475

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1021/acsnano.1c04814

UT WoS

000731055900001

Keywords in English

virus-like nanoparticlesRNA virusgenome releasecapsidcomputer simulationscoarse-grained modelcryo-EM

Abstract

V originále

Virus-like nanoparticles are protein shells similar to wild-type viruses, and both aim to deliver their content into a cell. Unfortunately, the release mechanism of their cargo/genome remains elusive. Pores on the symmetry axes were proposed to enable the slow release of the viral genome. In contrast, cryo-EM images showed that capsids of nonenveloped RNA viruses can crack open and rapidly release the genome. We combined in vitro cryo-EM observations of the genome release of three viruses with coarse-grained simulations of generic virus-like nanoparticles to investigate the cargo/genome release pathways. Simulations provided details on both slow and rapid release pathways, including the success rates of individual releases. Moreover, the simulated structures from the rapid release pathway were in agreement with the experiment. Slow release occurred when interactions between capsid subunits were long-ranged, and the cargo/genome was noncompact. In contrast, rapid release was preferred when the interaction range was short and/or the cargo/genome was compact. These findings indicate a design strategy of virus-like nanoparticles for drug delivery.

Links

GA20-20152S, research and development project

Name: Proteinová přitažlivost a selektivita pro buněčné membrány

Investor: Czech Science Foundation

GX19-25982X, research and development project

Name: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Czech Science Foundation

LL2007, research and development project

Name: Peptidoví zabijáci bakterií (Acronym: PeptideKillers)

Investor: Ministry of Education, Youth and Sports of the CR

LM2015085, research and development project

Name: CERIT Scientific Cloud (Acronym: CERIT-SC)

Investor: Ministry of Education, Youth and Sports of the CR, CERIT Scientific Cloud

90070, large research infrastructures

Name: IT4Innovations

90127, large research infrastructures

Name: CIISB II

Citovat

SUKENÍK, Lukáš, Liya MUKHAMEDOVA, Michaela PROCHÁZKOVÁ, Karel ŠKUBNÍK, Pavel PLEVKA and Robert VÁCHA. Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation. ACS Nano. Washington, D.C.: American Chemical Society, 2021, vol. 15, No 12, p. 19233-19243. ISSN 1936-0851. Available from: https://dx.doi.org/10.1021/acsnano.1c04814.

@article{1815461,
   author = {Sukeník, Lukáš and Mukhamedova, Liya and Procházková, Michaela and Škubník, Karel and Plevka, Pavel and Vácha, Robert},
   article_location = {Washington, D.C.},
   article_number = {12},
   doi = {http://dx.doi.org/10.1021/acsnano.1c04814},
   keywords = {virus-like nanoparticlesRNA virusgenome releasecapsidcomputer simulationscoarse-grained modelcryo-EM},
   language = {eng},
   issn = {1936-0851},
   journal = {ACS Nano},
   title = {Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation},
   url = {https://pubs.acs.org/doi/10.1021/acsnano.1c04814},
   volume = {15},
   year = {2021}
}

TY  - JOUR
ID  - 1815461
AU  - Sukeník, Lukáš - Mukhamedova, Liya - Procházková, Michaela - Škubník, Karel - Plevka, Pavel - Vácha, Robert
PY  - 2021
TI  - Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation
JF  - ACS Nano
VL  - 15
IS  - 12
SP  - 19233-19243
EP  - 19233-19243
PB  - American Chemical Society
SN  - 19360851
KW  - virus-like nanoparticlesRNA virusgenome releasecapsidcomputer simulationscoarse-grained modelcryo-EM
UR  - https://pubs.acs.org/doi/10.1021/acsnano.1c04814
N2  - Virus-like nanoparticles are protein shells similar to wild-type viruses, and both aim to deliver their content into a cell. Unfortunately, the release mechanism of their cargo/genome remains elusive. Pores on the symmetry axes were proposed to enable the slow release of the viral genome. In contrast, cryo-EM images showed that capsids of nonenveloped RNA viruses can crack open and rapidly release the genome. We combined in vitro cryo-EM observations of the genome release of three viruses with coarse-grained simulations of generic virus-like nanoparticles to investigate the cargo/genome release pathways. Simulations provided details on both slow and rapid release pathways, including the success rates of individual releases. Moreover, the simulated structures from the rapid release pathway were in agreement with the experiment. Slow release occurred when interactions between capsid subunits were long-ranged, and the cargo/genome was noncompact. In contrast, rapid release was preferred when the interaction range was short and/or the cargo/genome was compact. These findings indicate a design strategy of virus-like nanoparticles for drug delivery.
ER  -

SUKENÍK, Lukáš, Liya MUKHAMEDOVA, Michaela PROCHÁZKOVÁ, Karel ŠKUBNÍK, Pavel PLEVKA and Robert VÁCHA. Cargo Release from Nonenveloped Viruses and Virus-like Nanoparticles: Capsid Rupture or Pore Formation. \textit{ACS Nano}. Washington, D.C.: American Chemical Society, 2021, vol.~15, No~12, p.~19233-19243. ISSN~1936-0851. Available from: https://dx.doi.org/10.1021/acsnano.1c04814.

Detailed Information on Publication Record