2022
Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
HRUŠKA, Pavel; Jan KUČERA; Matej PEKAŘ; Pavol HOLÉCZY; Miloslav MAZUR et. al.Basic information
Original name
Proteomic Signatures of Human Visceral and Subcutaneous Adipocytes
Authors
HRUŠKA, Pavel (203 Czech Republic, belonging to the institution); Jan KUČERA (203 Czech Republic, belonging to the institution); Matej PEKAŘ (703 Slovakia, belonging to the institution); Pavol HOLÉCZY (703 Slovakia); Miloslav MAZUR (203 Czech Republic); Marek BUZGA (203 Czech Republic); Daniela KURUCZOVÁ (703 Slovakia, belonging to the institution); Peter LENÁRT (703 Slovakia, belonging to the institution); Jana FIALOVÁ KUČEROVÁ (203 Czech Republic, belonging to the institution); David POTĚŠIL (203 Czech Republic, belonging to the institution); Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution) and Julie DOBROVOLNÁ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Journal of Clinical Endocrinology and Metabolism, Endocrine Society, 2022, 0021-972X
Other information
Language
English
Type of outcome
Article in a journal
Field of Study
30202 Endocrinology and metabolism
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
is not subject to a state or trade secret
References:
Impact factor
Impact factor: 5.800
RIV identification code
RIV/00216224:14310/22:00125133
Organization unit
Faculty of Science
UT WoS
000756897900013
EID Scopus
2-s2.0-85124850550
Keywords in English
adipose tissue; visceral adipose tissue; subcutaneous adipose tissue; obesity; adipocytes; metabolism
Tags
International impact, Reviewed
Changed: 11/6/2025 07:50, Mgr. Michaela Hylsová, Ph.D.
Abstract
In the original language
Context: Adipose tissue distribution is a key factor influencing metabolic health and risk in obesity-associated comorbidities. Objective: Here we aim to compare the proteomic profiles of mature adipocytes from different depots. Methods: Abdominal subcutaneous (SA) and omental visceral adipocytes (VA) were isolated from paired adipose tissue biopsies obtained during bariatric surgery on 19 severely obese women (body mass index > 30 kg/m(2)) and analyzed using state-of-the-art mass spectrometry. Differential expression analysis and weighted gene co-expression network analysis (WGCNA) were performed to investigate proteome signature properties and to examine a possible association of the protein expression with the clinical data. Results: We identified 3686 protein groups and found 1140 differentially expressed proteins (adj. P value < 0.05), of which 576 proteins were upregulated in SA and 564 in VA samples. We provide a global protein profile of abdominal SA and omental VA, present the most differentially expressed pathways and processes distinguishing SA from VA, and correlate them with clinical and body composition data. We show that SA are significantly more active in processes linked to vesicular transport and secretion, and to increased lipid metabolism activity. Conversely, the expression of proteins involved in the mitochondria! energy metabolism and translational or biosynthetic activity is higher in VA. Conclusion: Our analysis represents a valuable resource of protein expression profiles in abdominal SA and omental VA, highlighting key differences in their role in obesity.
Links
| EF15_003/0000469, research and development project |
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| EF17_043/0009632, research and development project |
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| LM2018140, research and development project |
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| MUNI/A/1246/2020, interní kód MU |
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| MUNI/A/1307/2019, interní kód MU |
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| MUNI/A/1615/2020, interní kód MU |
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| MUNI/A/1698/2020, interní kód MU |
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| 857560, interní kód MU (CEP code: EF17_043/0009632) |
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| 90121, large research infrastructures |
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| 90127, large research infrastructures |
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