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@article{1816729, author = {Bouchal, Tomáš and Durník, Ivo and Kulhánek, Petr}, article_number = {12}, doi = {http://dx.doi.org/10.1021/acs.jcim.1c00731}, keywords = {Free energy; Genetics; Chemical structure; Intercalation; Nucleic acid structure}, language = {eng}, issn = {1549-9596}, journal = {Journal of Chemical Information and Modeling}, title = {Bending of Canonical and G/T Mismatched DNAs}, url = {https://pubs.acs.org/doi/10.1021/acs.jcim.1c00731}, volume = {61}, year = {2021} }
TY - JOUR ID - 1816729 AU - Bouchal, Tomáš - Durník, Ivo - Kulhánek, Petr PY - 2021 TI - Bending of Canonical and G/T Mismatched DNAs JF - Journal of Chemical Information and Modeling VL - 61 IS - 12 SP - 6000-6011 EP - 6000-6011 PB - American Chemical Society SN - 15499596 KW - Free energy KW - Genetics KW - Chemical structure KW - Intercalation KW - Nucleic acid structure UR - https://pubs.acs.org/doi/10.1021/acs.jcim.1c00731 N2 - Mismatched base pairs alter the flexibility and intrinsic curvature of DNA. The role of such DNA features is not fully understood in the mismatch repair pathway. MutS/DNA complexes exhibit DNA bending, PHE intercalation, and changes of base-pair parameters near the mismatch. Recently, we have shown that base-pair opening in the absence of MutS can discriminate mismatches from canonical base pairs better than DNA bending. However, DNA bending in the absence of MutS was found to be rather challenging to describe correctly. Here, we present a computational study on the DNA bending of canonical and G/T mismatched DNAs. Five types of geometric parameters covering template-based bending toward the experimental DNA structure, global, and local geometry parameters were employed in biased molecular dynamics in the absence of MutS. None of these parameters showed higher discrimination than the base-pair opening. Only roll could induce a sharply localized bending of DNA as observed in the experimental MutS/DNA structure. Further, we demonstrated that the intercalation of benzene mimicking PHE decreases the energetic cost of DNA bending without any effect on mismatch discrimination. ER -
BOUCHAL, Tomáš, Ivo DURNÍK and Petr KULHÁNEK. Bending of Canonical and G/T Mismatched DNAs. \textit{Journal of Chemical Information and Modeling}. American Chemical Society, 2021, vol.~61, No~12, p.~6000-6011. ISSN~1549-9596. Available from: https://dx.doi.org/10.1021/acs.jcim.1c00731.
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