Species-specific PCR assay for the detection of Babesia
odocoilei

J 2021

Species-specific PCR assay for the detection of Babesia odocoilei

BURGESS, Hilary J., Betty P. LOCKERBIE, Lisanework E. AYALEW, Antonia DIBERNARDO, Kristýna HRAZDILOVÁ et. al.

Basic information

Original name

Species-specific PCR assay for the detection of Babesia odocoilei

Authors

BURGESS, Hilary J. (guarantor), Betty P. LOCKERBIE, Lisanework E. AYALEW, Antonia DIBERNARDO, Kristýna HRAZDILOVÁ, David MODRÝ (203 Czech Republic, belonging to the institution) and Trent K. BOLLINGER

Edition

Journal of Veterinary Diagnostic Investigation, SAGE Publications Inc. 2021, 1040-6387

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

40300 4.3 Veterinary science

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.569

RIV identification code

RIV/00216224:14310/21:00123524

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1177/10406387211032927

UT WoS

000703496400001

Keywords in English

Babesia odocoilei; cervids; PCR; specificity

Abstract

V originále

We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.

Links

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

Citovat

BURGESS, Hilary J., Betty P. LOCKERBIE, Lisanework E. AYALEW, Antonia DIBERNARDO, Kristýna HRAZDILOVÁ, David MODRÝ and Trent K. BOLLINGER. Species-specific PCR assay for the detection of Babesia odocoilei. Journal of Veterinary Diagnostic Investigation. SAGE Publications Inc., 2021, vol. 33, No 6, p. 1188-1192. ISSN 1040-6387. Available from: https://dx.doi.org/10.1177/10406387211032927.

@article{1817118,
   author = {Burgess, Hilary J. and Lockerbie, Betty P. and Ayalew, Lisanework E. and Dibernardo, Antonia and Hrazdilová, Kristýna and Modrý, David and Bollinger, Trent K.},
   article_number = {6},
   doi = {http://dx.doi.org/10.1177/10406387211032927},
   keywords = {Babesia odocoilei; cervids; PCR; specificity},
   language = {eng},
   issn = {1040-6387},
   journal = {Journal of Veterinary Diagnostic Investigation},
   title = {Species-specific PCR assay for the detection of Babesia odocoilei},
   url = {https://doi.org/10.1186/s13071-017-2064-1},
   volume = {33},
   year = {2021}
}

TY  - JOUR
ID  - 1817118
AU  - Burgess, Hilary J. - Lockerbie, Betty P. - Ayalew, Lisanework E. - Dibernardo, Antonia - Hrazdilová, Kristýna - Modrý, David - Bollinger, Trent K.
PY  - 2021
TI  - Species-specific PCR assay for the detection of Babesia odocoilei
JF  - Journal of Veterinary Diagnostic Investigation
VL  - 33
IS  - 6
SP  - 1188-1192
EP  - 1188-1192
PB  - SAGE Publications Inc.
SN  - 10406387
KW  - Babesia odocoilei
KW  - cervids
KW  - PCR
KW  - specificity
UR  - https://doi.org/10.1186/s13071-017-2064-1
N2  - We developed a PCR assay for the detection of Babesia odocoilei based on the 18S rRNA gene. Multiple specimens of B. odocoilei were examined, and the assay consistently produced a small specific PCR product of 306 bp. The PCR assay was also challenged with DNA from 13 other Babesia species and 2 Theileria species, originating from 10 different host species; however, nonspecific DNA amplification and multiple banding patterns were observed, and the amplicon banding patterns varied between different isolates of the same species. Sensitivity was determined to be 6.4 pg of DNA, and an estimated 0.0001% parasitism. This assay can be utilized for species-specific differential detection of B. odocoilei.
ER  -

BURGESS, Hilary J., Betty P. LOCKERBIE, Lisanework E. AYALEW, Antonia DIBERNARDO, Kristýna HRAZDILOVÁ, David MODRÝ and Trent K. BOLLINGER. Species-specific PCR assay for the detection of Babesia odocoilei. \textit{Journal of Veterinary Diagnostic Investigation}. SAGE Publications Inc., 2021, vol.~33, No~6, p.~1188-1192. ISSN~1040-6387. Available from: https://dx.doi.org/10.1177/10406387211032927.

Detailed Information on Publication Record