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@article{1819298,
author = {Kashkan, Ivan and Hrtyan, Mónika and Retzer, Katarzyna and Humpolíčková, Jana and Jayasree, Aswathy and Filepová, Roberta and Vondráková, Zuzana and Simon, Sibu and Rombaut, Debbie and Jacobs, Thomas B. and Frilander, Mikko J. and Hejátko, Jan and Friml, Jiří and Petrášek, Jan and Růžička, Kamil},
article_number = {1},
doi = {http://dx.doi.org/10.1111/nph.17792},
keywords = {alternative splicing; Arabidopsis thaliana; auxin; auxin transport; FRAP; PINs; plant development; RNA processing},
language = {eng},
issn = {0028-646X},
journal = {New Phytologist},
title = {Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana},
url = {https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792},
volume = {233},
year = {2022}
}
TY - JOUR
ID - 1819298
AU - Kashkan, Ivan - Hrtyan, Mónika - Retzer, Katarzyna - Humpolíčková, Jana - Jayasree, Aswathy - Filepová, Roberta - Vondráková, Zuzana - Simon, Sibu - Rombaut, Debbie - Jacobs, Thomas B. - Frilander, Mikko J. - Hejátko, Jan - Friml, Jiří - Petrášek, Jan - Růžička, Kamil
PY - 2022
TI - Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
JF - New Phytologist
VL - 233
IS - 1
SP - 329-343
EP - 329-343
PB - Wiley
SN - 0028646X
KW - alternative splicing
KW - Arabidopsis thaliana
KW - auxin
KW - auxin transport
KW - FRAP
KW - PINs
KW - plant development
KW - RNA processing
UR - https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792
N2 - Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.
ER -
KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE, Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO, Jiří FRIML, Jan PETRÁŠEK and Kamil RŮŽIČKA. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. \textit{New Phytologist}. Wiley, 2022, vol.~233, No~1, p.~329-343. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.17792.
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