Mutually opposing activity of PIN7 splicing isoforms is
required for auxin-mediated tropic responses in Arabidopsis
thaliana

J 2022

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE et. al.

Basic information

Original name

Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana

Authors

KASHKAN, Ivan (112 Belarus, belonging to the institution), Mónika HRTYAN (348 Hungary, belonging to the institution), Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE (356 India, belonging to the institution), Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO (203 Czech Republic, belonging to the institution), Jiří FRIML, Jan PETRÁŠEK and Kamil RŮŽIČKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

New Phytologist, Wiley, 2022, 0028-646X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 9.400

RIV identification code

RIV/00216224:14740/22:00125152

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1111/nph.17792

UT WoS

000714678100001

Keywords in English

alternative splicing; Arabidopsis thaliana; auxin; auxin transport; FRAP; PINs; plant development; RNA processing

Abstract

V originále

Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.

Links

EF16_013/0001775, research and development project

Name: Modernizace a podpora výzkumných aktivit národní infrastruktury pro biologické a medicínské zobrazování Czech-BioImaging

EF16_019/0000738, research and development project

Name: Centrum experimentální biologie rostlin

GA16-26428S, research and development project

Name: Alternativní sestřih jako regulátor auxinového transportu

Investor: Czech Science Foundation

GA18-26981S, research and development project

Name: Genetické studie k objasnění molekulárního mechanizmu účinku strigolaktonů v kořeni

Investor: Czech Science Foundation

90129, large research infrastructures

Name: Czech-BioImaging II

Citovat

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE, Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO, Jiří FRIML, Jan PETRÁŠEK and Kamil RŮŽIČKA. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. New Phytologist. Wiley, 2022, vol. 233, No 1, p. 329-343. ISSN 0028-646X. Available from: https://dx.doi.org/10.1111/nph.17792.

@article{1819298,
   author = {Kashkan, Ivan and Hrtyan, Mónika and Retzer, Katarzyna and Humpolíčková, Jana and Jayasree, Aswathy and Filepová, Roberta and Vondráková, Zuzana and Simon, Sibu and Rombaut, Debbie and Jacobs, Thomas B. and Frilander, Mikko J. and Hejátko, Jan and Friml, Jiří and Petrášek, Jan and Růžička, Kamil},
   article_number = {1},
   doi = {http://dx.doi.org/10.1111/nph.17792},
   keywords = {alternative splicing; Arabidopsis thaliana; auxin; auxin transport; FRAP; PINs; plant development; RNA processing},
   language = {eng},
   issn = {0028-646X},
   journal = {New Phytologist},
   title = {Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana},
   url = {https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792},
   volume = {233},
   year = {2022}
}

TY  - JOUR
ID  - 1819298
AU  - Kashkan, Ivan - Hrtyan, Mónika - Retzer, Katarzyna - Humpolíčková, Jana - Jayasree, Aswathy - Filepová, Roberta - Vondráková, Zuzana - Simon, Sibu - Rombaut, Debbie - Jacobs, Thomas B. - Frilander, Mikko J. - Hejátko, Jan - Friml, Jiří - Petrášek, Jan - Růžička, Kamil
PY  - 2022
TI  - Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana
JF  - New Phytologist
VL  - 233
IS  - 1
SP  - 329-343
EP  - 329-343
PB  - Wiley
SN  - 0028646X
KW  - alternative splicing
KW  - Arabidopsis thaliana
KW  - auxin
KW  - auxin transport
KW  - FRAP
KW  - PINs
KW  - plant development
KW  - RNA processing
UR  - https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.17792
N2  - Advanced transcriptome sequencing has revealed that the majority of eukaryotic genes undergo alternative splicing (AS). Nonetheless, little effort has been dedicated to investigating the functional relevance of particular splicing events, even those in the key developmental and hormonal regulators. Combining approaches of genetics, biochemistry and advanced confocal microscopy, we describe the impact of alternative splicing on the PIN7 gene in the model plant Arabidopsis thaliana. PIN7 encodes a polarly localized transporter for the phytohormone auxin and produces two evolutionarily conserved transcripts, PIN7a and PIN7b. PIN7a and PIN7b, differing in a four amino acid stretch, exhibit almost identical expression patterns and subcellular localization. We reveal that they are closely associated and mutually influence each other's mobility within the plasma membrane. Phenotypic complementation tests indicate that the functional contribution of PIN7b per se is minor, but it markedly reduces the prominent PIN7a activity, which is required for correct seedling apical hook formation and auxin-mediated tropic responses. Our results establish alternative splicing of the PIN family as a conserved, functionally relevant mechanism, revealing an additional regulatory level of auxin-mediated plant development.
ER  -

KASHKAN, Ivan, Mónika HRTYAN, Katarzyna RETZER, Jana HUMPOLÍČKOVÁ, Aswathy JAYASREE, Roberta FILEPOVÁ, Zuzana VONDRÁKOVÁ, Sibu SIMON, Debbie ROMBAUT, Thomas B. JACOBS, Mikko J. FRILANDER, Jan HEJÁTKO, Jiří FRIML, Jan PETRÁŠEK and Kamil RŮŽIČKA. Mutually opposing activity of PIN7 splicing isoforms is required for auxin-mediated tropic responses in Arabidopsis thaliana. \textit{New Phytologist}. Wiley, 2022, vol.~233, No~1, p.~329-343. ISSN~0028-646X. Available from: https://dx.doi.org/10.1111/nph.17792.

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