Structure elucidation of multicolor emissive graphene quantum
dots towards cell guidance

J 2022

Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance

GOMEZ PEREZ, Inmaculada Jennifer, Manuel Vázquez SULLEIRO, Anna DOLEČKOVÁ, Naděžda PIZÚROVÁ, Jiřina MEDALOVÁ et. al.

Basic information

Original name

Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance

Authors

GOMEZ PEREZ, Inmaculada Jennifer (724 Spain, belonging to the institution), Manuel Vázquez SULLEIRO, Anna DOLEČKOVÁ (203 Czech Republic, belonging to the institution), Naděžda PIZÚROVÁ (203 Czech Republic), Jiřina MEDALOVÁ (203 Czech Republic, belonging to the institution), Antonín BEDNAŘÍK (203 Czech Republic, belonging to the institution), Jan PREISLER (203 Czech Republic, belonging to the institution), David NEČAS (203 Czech Republic) and Lenka ZAJÍČKOVÁ (203 Czech Republic, belonging to the institution)

Edition

Materials Chemistry Frontiers, Cambridge, Royal Society of Chemistry, 2022, 2052-1537

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10303 Particles and field physics

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 7.000

RIV identification code

RIV/00216224:14310/22:00119559

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1039/d1qm01126j

UT WoS

000730146400001

Keywords in English

FACILE SYNTHESIS; CARBON DOTS; 2ND WINDOW; PHOTOLUMINESCENCE; NANODOTS; OXIGEN; NITROGEN; YIELD; MOLECULES; REDUCTION

Abstract

V originále

Graphene quantum dots (GQDs) can become excellent bioimaging tools when tuned to emit at larger wavelengths due to the minimal tissue absorbance and emission in this range. Tuning the GQD structure can help but understanding the chemical structure responsible for their properties remains challenging. Herein, we elucidated the structure of GQDs synthesized from glucose and ammonium hydroxide using a fast microwave-assisted hydrothermal protocol. Remarkably, these GQDs exhibited emission from the NUV-Vis up to the NIR range. The structure and chemical composition were elucidated using advanced NMR techniques, such as two-dimensional nuclear magnetic resonance, combined with traditional spectroscopy and electron microscopy. The graphitic core composed of pyrazines presented localized defects and lower rotation mobility compared with their edges that were mainly formed by hydroxyl, acid, and amine functional groups, which paved the way for the observed multicolor red-shifted fluorescence emission. Confocal laser scanning microscopy revealed functional cell imaging in a wide spectral range of fluorescence from bright purple to red, confirming the uptake of GQDs by the cells without any observable toxicity. The non-cytotoxicity was further proved by the chemiluminescence cell viability adenosine triphosphate (ATP) assay. Combined with the tunable GQD emission, it gives them the potential to act as bioimaging carriers starting a new phase for their use in vivo.

Links

EF17_043/0009632, research and development project

Name: CETOCOEN Excellence

EF20_079/0017045, research and development project

Name: MSCAfellow4@MUNI

GA21-12262S, research and development project

Name: Nanočásticové sondy pro zobrazovací hmotnostní spektrometrii

Investor: Czech Science Foundation

LM2018121, research and development project

Name: Výzkumná infrastruktura RECETOX (Acronym: RECETOX RI)

Investor: Ministry of Education, Youth and Sports of the CR, RECETOX RI

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

857560, interní kód MU
(CEP code: EF17_043/0009632)

Name: CETOCOEN Excellence (Acronym: CETOCOEN Excellence)

Investor: European Union, Spreading excellence and widening participation

90110, large research infrastructures

Name: CzechNanoLab

Citovat

GOMEZ PEREZ, Inmaculada Jennifer, Manuel Vázquez SULLEIRO, Anna DOLEČKOVÁ, Naděžda PIZÚROVÁ, Jiřina MEDALOVÁ, Antonín BEDNAŘÍK, Jan PREISLER, David NEČAS and Lenka ZAJÍČKOVÁ. Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance. Materials Chemistry Frontiers. Cambridge: Royal Society of Chemistry, 2022, vol. 6, No 2, p. 145-154. ISSN 2052-1537. Available from: https://dx.doi.org/10.1039/d1qm01126j.

@article{1820330,
   author = {Gomez Perez, Inmaculada Jennifer and Sulleiro, Manuel Vázquez and Dolečková, Anna and Pizúrová, Naděžda and Medalová, Jiřina and Bednařík, Antonín and Preisler, Jan and Nečas, David and Zajíčková, Lenka},
   article_location = {Cambridge},
   article_number = {2},
   doi = {http://dx.doi.org/10.1039/d1qm01126j},
   keywords = {FACILE SYNTHESIS; CARBON DOTS; 2ND WINDOW; PHOTOLUMINESCENCE; NANODOTS; OXIGEN; NITROGEN; YIELD; MOLECULES; REDUCTION},
   language = {eng},
   issn = {2052-1537},
   journal = {Materials Chemistry Frontiers},
   title = {Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance},
   url = {https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J},
   volume = {6},
   year = {2022}
}

TY  - JOUR
ID  - 1820330
AU  - Gomez Perez, Inmaculada Jennifer - Sulleiro, Manuel Vázquez - Dolečková, Anna - Pizúrová, Naděžda - Medalová, Jiřina - Bednařík, Antonín - Preisler, Jan - Nečas, David - Zajíčková, Lenka
PY  - 2022
TI  - Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance
JF  - Materials Chemistry Frontiers
VL  - 6
IS  - 2
SP  - 145-154
EP  - 145-154
PB  - Royal Society of Chemistry
SN  - 20521537
KW  - FACILE SYNTHESIS
KW  - CARBON DOTS
KW  - 2ND WINDOW
KW  - PHOTOLUMINESCENCE
KW  - NANODOTS
KW  - OXIGEN
KW  - NITROGEN
KW  - YIELD
KW  - MOLECULES
KW  - REDUCTION
UR  - https://pubs.rsc.org/en/content/articlelanding/2022/QM/D1QM01126J
N2  - Graphene quantum dots (GQDs) can become excellent bioimaging tools when tuned to emit at larger wavelengths due to the minimal tissue absorbance and emission in this range. Tuning the GQD structure can help but understanding the chemical structure responsible for their properties remains challenging. Herein, we elucidated the structure of GQDs synthesized from glucose and ammonium hydroxide using a fast microwave-assisted hydrothermal protocol. Remarkably, these GQDs exhibited emission from the NUV-Vis up to the NIR range. The structure and chemical composition were elucidated using advanced NMR techniques, such as two-dimensional nuclear magnetic resonance, combined with traditional spectroscopy and electron microscopy. The graphitic core composed of pyrazines presented localized defects and lower rotation mobility compared with their edges that were mainly formed by hydroxyl, acid, and amine functional groups, which paved the way for the observed multicolor red-shifted fluorescence emission. Confocal laser scanning microscopy revealed functional cell imaging in a wide spectral range of fluorescence from bright purple to red, confirming the uptake of GQDs by the cells without any observable toxicity. The non-cytotoxicity was further proved by the chemiluminescence cell viability adenosine triphosphate (ATP) assay. Combined with the tunable GQD emission, it gives them the potential to act as bioimaging carriers starting a new phase for their use in vivo.
ER  -

GOMEZ PEREZ, Inmaculada Jennifer, Manuel Vázquez SULLEIRO, Anna DOLEČKOVÁ, Naděžda PIZÚROVÁ, Jiřina MEDALOVÁ, Antonín BEDNAŘÍK, Jan PREISLER, David NEČAS and Lenka ZAJÍČKOVÁ. Structure elucidation of multicolor emissive graphene quantum dots towards cell guidance. \textit{Materials Chemistry Frontiers}. Cambridge: Royal Society of Chemistry, 2022, vol.~6, No~2, p.~145-154. ISSN~2052-1537. Available from: https://dx.doi.org/10.1039/d1qm01126j.

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