SLANINA, Peter, Julie ŠTÍCHOVÁ, Jiří LITZMAN, Zita CHOVANCOVÁ, Jan FRIČ and Marcela VLKOVÁ. IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID (Intravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID). In 11th International Conference Analytical Cytometry. 2021. ISBN 978-80-88214-26-7.
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Basic information
Original name IIntravenous immunoglobulin modulates the number and function of low density neutrophils in patients with CVID
Name in Czech Intravenózní imunoglobuliny ovlivňují počet a funkci neutrofilů s nízkou denzitou u pacientů s CVID
Authors SLANINA, Peter (703 Slovakia, belonging to the institution), Julie ŠTÍCHOVÁ (203 Czech Republic, belonging to the institution), Jiří LITZMAN (203 Czech Republic, belonging to the institution), Zita CHOVANCOVÁ (203 Czech Republic, belonging to the institution), Jan FRIČ (203 Czech Republic, belonging to the institution) and Marcela VLKOVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition 11th International Conference Analytical Cytometry, 2021.
Other information
Original language English
Type of outcome Conference abstract
Field of Study 30102 Immunology
Country of publisher Czech Republic
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14110/21:00123777
Organization unit Faculty of Medicine
ISBN 978-80-88214-26-7
Keywords (in Czech) Běžný variabilní imunodeficit; neutrofily s nízkou denzitou
Keywords in English Common variable immunodeficiency; Low Density Neutrophils
Tags International impact, Reviewed
Changed by Changed by: Mgr. Marcela Vlková, Ph.D., učo 67941. Changed: 17/1/2022 12:33.
Abstract
Common variable immunodeficiency disorder (CVID) is the most common form of clinically significant primary immunodeficiency. Majority of patients receive intravenous immunoglobulin (IVIg) replacement therapy with potent immunomodulatory properties on individual components of the immune system. CVID is associated with chronic granulocytic activation and an increased percentage of Low Density Neutrophils (LDN) observed in the layer of peripheral blood mononuclear cells (PBMC). Changes in the percentage of LDN in PBMC and expression of their surface markers were studied in 25 CVID patients and 27 healthy donors (HD) in a whole blood sample and after in-vitro stimulation of whole blood with IVIg. We confirmed an increased number of LDN in the PBMC layer in a fresh blood sample from CVID patients. The LDN of patients and a HD group consisted of mature and immature neutrophils distinguished based on the expression of CD10 and CD16. Stimulation of whole blood with IVIg caused an increase of LDN in both CVID patients and HD. This enhancement in LDN was caused by mature neutrophils CD16+CD10+ in both groups. The suppressive effect of induced LDN on T-cell proliferation was confirmed. Flow cytometry measurements revealed altered expression of CD274 and CD10 on mature LDN of CVID patients. The addition of IVIg inhibited the expression of CD11b on the mature LDN in both experimental groups. These results indicate that IVIg supports the formation of LDN, which can negatively affect the immune response and can significantly contribute to the chronic inflammation observed in patients with CVID.
Links
MUNI/A/1244/2021, interní kód MUName: Vrozená imunita a její abnormality v rozvoji imunopatologických stavů
Investor: Masaryk University
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