DUBOVÝ, Petr, Karolína BRETOVÁ, Viktorie SVOBODOVÁ, Anna BAGÓ a Pere BOADAS-VAELLO. Fractalkine/CX3CL1 and its receptor CX3CR1 in the anterior cingulate cortex of the experimental model of neuropathic pain. 2021.
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Základní údaje
Originální název Fractalkine/CX3CL1 and its receptor CX3CR1 in the anterior cingulate cortex of the experimental model of neuropathic pain
Autoři DUBOVÝ, Petr, Karolína BRETOVÁ, Viktorie SVOBODOVÁ, Anna BAGÓ a Pere BOADAS-VAELLO.
Vydání 2021.
Další údaje
Originální jazyk angličtina
Typ výsledku Konferenční abstrakt
Stát vydavatele Česká republika
Utajení není předmětem státního či obchodního tajemství
WWW URL
Organizační jednotka Lékařská fakulta
Změnil Změnil: prof. RNDr. Petr Dubový, CSc., učo 698. Změněno: 20. 1. 2022 12:03.
Anotace
The anterior cingulate cortex (ACC) mediates the affective component of neuropathic pain responses. Given the strategic functions of chemokines in chemoattraction, the study of the functional involvement of CX3CL1/CX3CR1 signaling in the brain was focused on neuron-microglia interactions, neglecting the signaling role in neurons by an autocrine manner. Female adult CD1 Swiss mice were operated on the spinal nerve injury (SCI) by contusion using weight drop apparatus following dorsal laminectomy at T8-T9 (n=16). Naive and sham-operated mice were used as controls. Operated animals were left to survive for 6 or 12 weeks. Coronal cryostat sections through ACC (n=8) were immunostained for cellular detection of CX3CL1/CX3CR1, CathepsinS, and ADAM17 under the same conditions. Immunofluorescence (IF) intensity was measured using our standard protocol. Moreover, CX3CL1, CX3CR1, and CathepsinS protein levels were assessed using western blot analysis. We found that SCI induced increased IF for both CX3CL1 and CX3CR1 in the ACC neurons of the lamina 2/3 when compared with controls. The increased protein levels were verified by western blot analysis. CathepsinS-IF and ADAM17-IF were also observed in the ACC neurons while western blot failed to detect CathepsinS protein levels. The biological activity of CX3CL1 is regulated by the conversion of a membrane integrated into a soluble form. Our results of immunofluorescence staining suggested that neuronal sCX3CL1 in ACC after SCI may act in an autocrine manner to be involved in neuroprotection. Supported by MUNI/A/1520/2020 and La MARATÓ de TV3 Foundation (201705.30.31).
Návaznosti
MUNI/A/1520/2020, interní kód MUNázev: Změny ve strukturách nervové soustavy v reakci na poškození
Investor: Masarykova univerzita, Změny ve strukturách nervové soustavy v reakci na poškození
VytisknoutZobrazeno: 28. 3. 2024 21:18