p38-MAPK-mediated translation regulation during early
blastocyst development is required for primitive endoderm
differentiation in mice

J 2021

p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

BORA, Pablo, Lenka GAHUROVÁ, Tomáš MAŠEK, Andrea HAUSEROVÁ, David POTĚŠIL et. al.

Basic information

Original name

p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice

Authors

BORA, Pablo (203 Czech Republic), Lenka GAHUROVÁ (203 Czech Republic), Tomáš MAŠEK (203 Czech Republic), Andrea HAUSEROVÁ (203 Czech Republic), David POTĚŠIL (203 Czech Republic, belonging to the institution), Denisa JANSOVÁ (203 Czech Republic), Andrej SUSOR, Zbyněk ZDRÁHAL (203 Czech Republic, guarantor, belonging to the institution), Anna AJDUK (616 Poland), Martin POSPÍŠEK (203 Czech Republic) and Alexander W. BRUCE

Edition

Communications Biology, 2021, 2399-3642

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10700 1.7 Other natural sciences

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 6.548

RIV identification code

RIV/00216224:14740/21:00123879

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1038/s42003-021-02290-z

UT WoS

000668739100001

Keywords in English

ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH

Abstract

V originále

Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

BORA, Pablo, Lenka GAHUROVÁ, Tomáš MAŠEK, Andrea HAUSEROVÁ, David POTĚŠIL, Denisa JANSOVÁ, Andrej SUSOR, Zbyněk ZDRÁHAL, Anna AJDUK, Martin POSPÍŠEK and Alexander W. BRUCE. p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice. Communications Biology. 2021, vol. 4, No 1, p. "788", 19 pp. ISSN 2399-3642. Available from: https://dx.doi.org/10.1038/s42003-021-02290-z.

@article{1824697,
   author = {Bora, Pablo and Gahurová, Lenka and Mašek, Tomáš and Hauserová, Andrea and Potěšil, David and Jansová, Denisa and Susor, Andrej and Zdráhal, Zbyněk and Ajduk, Anna and Pospíšek, Martin and Bruce, Alexander W.},
   article_number = {1},
   doi = {http://dx.doi.org/10.1038/s42003-021-02290-z},
   keywords = {ACTIVATED PROTEIN-KINASE; INNER CELL MASS; MAP KINASE; PREIMPLANTATION EMBRYO; MESSENGER-RNA; NA/K-ATPASE; 1A MYBBP1A; P38 MAPK; MOUSE; GROWTH},
   language = {eng},
   issn = {2399-3642},
   journal = {Communications Biology},
   title = {p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice},
   url = {https://www.nature.com/articles/s42003-021-02290-z.pdf},
   volume = {4},
   year = {2021}
}

TY  - JOUR
ID  - 1824697
AU  - Bora, Pablo - Gahurová, Lenka - Mašek, Tomáš - Hauserová, Andrea - Potěšil, David - Jansová, Denisa - Susor, Andrej - Zdráhal, Zbyněk - Ajduk, Anna - Pospíšek, Martin - Bruce, Alexander W.
PY  - 2021
TI  - p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice
JF  - Communications Biology
VL  - 4
IS  - 1
SP  - "788"
EP  - "788"
SN  - 23993642
KW  - ACTIVATED PROTEIN-KINASE
KW  - INNER CELL MASS
KW  - MAP KINASE
KW  - PREIMPLANTATION EMBRYO
KW  - MESSENGER-RNA
KW  - NA/K-ATPASE
KW  - 1A MYBBP1A
KW  - P38 MAPK
KW  - MOUSE
KW  - GROWTH
UR  - https://www.nature.com/articles/s42003-021-02290-z.pdf
N2  - Successful specification of the two mouse blastocyst inner cell mass (ICM) lineages (the primitive endoderm (PrE) and epiblast) is a prerequisite for continued development and requires active fibroblast growth factor 4 (FGF4) signaling. Previously, we identified a role for p38 mitogen-activated protein kinases (p38-MAPKs) during PrE differentiation, but the underlying mechanisms have remained unresolved. Here, we report an early blastocyst window of p38-MAPK activity that is required to regulate ribosome-related gene expression, rRNA precursor processing, polysome formation and protein translation. We show that p38-MAPK inhibition-induced PrE phenotypes can be partially rescued by activating the translational regulator mTOR. However, similar PrE phenotypes associated with extracellular signal-regulated kinase (ERK) pathway inhibition targeting active FGF4 signaling are not affected by mTOR activation. These data indicate a specific role for p38-MAPKs in providing a permissive translational environment during mouse blastocyst PrE differentiation that is distinct from classically reported FGF4-based mechanisms. Bora et al. show that an early blastocyst window of p38-MAPK activity regulates ribosome-related gene expression, rRNA precursor processing, polysome formation, and protein translation. This study suggests a distinct role of p38-MAPKs for providing a permissive translational environment during mouse blastocyst primitive endoderm differentiation.
ER  -

BORA, Pablo, Lenka GAHUROVÁ, Tomáš MAŠEK, Andrea HAUSEROVÁ, David POTĚŠIL, Denisa JANSOVÁ, Andrej SUSOR, Zbyněk ZDRÁHAL, Anna AJDUK, Martin POSPÍŠEK and Alexander W. BRUCE. p38-MAPK-mediated translation regulation during early blastocyst development is required for primitive endoderm differentiation in mice. \textit{Communications Biology}. 2021, vol.~4, No~1, p.~''788'', 19 pp. ISSN~2399-3642. Available from: https://dx.doi.org/10.1038/s42003-021-02290-z.

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