In vivo and in vitro cell-based model of lung adenocarcinoma
from patients with pleural effusion

J 2021

In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion

POPOVIČ, Mikuláš, Yiling LIU, Erika LATTOVÁ, Dean MANN, Sabrina CURRELI et. al.

Basic information

Original name

In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion

Authors

POPOVIČ, Mikuláš (840 United States of America), Yiling LIU (840 United States of America), Erika LATTOVÁ (703 Slovakia, guarantor, belonging to the institution), Dean MANN (840 United States of America), Sabrina CURRELI (840 United States of America), Zbyněk ZDRÁHAL (203 Czech Republic, belonging to the institution), Martin EDELMAN (840 United States of America) and Joseph BRYANT (840 United States of America)

Edition

Neoplasma, Slovenská akademie vied, 2021, 0028-2685

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30204 Oncology

Country of publisher

Slovakia

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 3.409

RIV identification code

RIV/00216224:14740/21:00123881

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.4149/neo_2021_200906N953

UT WoS

000662144000005

Keywords in English

cell-based model of LAC; lung adenocarcinoma; growth pattern conversion; OCT-4; SOX-2; NANOG; pluripotent genes

Abstract

V originále

Lung adenocarcinoma (LAC) is a common and aggressive form of lung cancer that is increasing in incidence among never smokers at a younger age. Current treatment of patients with LAC is insufficient and there is a need for identification of effective biomarkers and development of therapeutic targets. These demands require also unproved models for in vivo and in vitro experimentation. In this study, we describe the establishment of two LAC cell lines, named LuCa-3 and LuCa-6. Both were derived from pleural effusion (PE) cells of LAC patients (13 and 16) and readily propagated as tumor xenografts in immunodeficient mice. PE cells from the patient L6 exhibited also the capacity for in vitro growth and were cultured in two forms: (i) as a suspension growing cell population, labeled LuCa-6S, composed of non-clumping single cells; and (ii) as a monolayer-like culture, labeled LuCa-6A, exhibiting tight cell-to-cell and to culture surface adherence. Unique features of these two sublines and their cell clones are the capacity to convert from a non-clumping single-cell suspension into the adherent growth pattern and vice versa. Immunostaining of patients' tumor tissue xenografts and cultured subline cells displayed markers specific for the phenotype of human LAC. LuCa-6S and LuCa-6A cells did not reveal a noticeable disparity in quantitative growth characteristics. However, a number of differences were detected between these two cell populations manifested in detection or intensities of antigen expressions on the cell surface (CD133, SFTPC) and in the nucleus (TTF-1) including pluripotcnt (OCT-4, SOX-2, NANOG) genes in cancer stem-like cells (CSCs). Dissimilarities between these two sublines were also detected in N-glycan profiles and in the sensitivity to natural killer cells. Salient features of these subline cell populations are responsiveness to selective upregulation of the pluripotent genes in subsets of CSCs via conversion of their growth patterns and/or by using culture stem media with growth factors. The described in vivo/in vitro model enables broader experimental approaches in studies of lung adenocarcinoma.

Links

90127, large research infrastructures

Name: CIISB II

Citovat

POPOVIČ, Mikuláš, Yiling LIU, Erika LATTOVÁ, Dean MANN, Sabrina CURRELI, Zbyněk ZDRÁHAL, Martin EDELMAN and Joseph BRYANT. In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion. Neoplasma. Slovenská akademie vied, 2021, vol. 68, No 3, p. 498-508. ISSN 0028-2685. Available from: https://dx.doi.org/10.4149/neo_2021_200906N953.

@article{1824758,
   author = {Popovič, Mikuláš and Liu, Yiling and Lattová, Erika and Mann, Dean and Curreli, Sabrina and Zdráhal, Zbyněk and Edelman, Martin and Bryant, Joseph},
   article_number = {3},
   doi = {http://dx.doi.org/10.4149/neo_2021_200906N953},
   keywords = {cell-based model of LAC; lung adenocarcinoma; growth pattern conversion; OCT-4; SOX-2; NANOG; pluripotent genes},
   language = {eng},
   issn = {0028-2685},
   journal = {Neoplasma},
   title = {In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion},
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   volume = {68},
   year = {2021}
}

TY  - JOUR
ID  - 1824758
AU  - Popovič, Mikuláš - Liu, Yiling - Lattová, Erika - Mann, Dean - Curreli, Sabrina - Zdráhal, Zbyněk - Edelman, Martin - Bryant, Joseph
PY  - 2021
TI  - In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion
JF  - Neoplasma
VL  - 68
IS  - 3
SP  - 498-508
EP  - 498-508
PB  - Slovenská akademie vied
SN  - 00282685
KW  - cell-based model of LAC
KW  - lung adenocarcinoma
KW  - growth pattern conversion
KW  - OCT-4
KW  - SOX-2
KW  - NANOG
KW  - pluripotent genes
UR  - http://www.elis.sk/index.php?page=shop.product_details&flypage=flypage.tpl&product_id=7102&category_id=172&option=com_virtuemart&vmcchk=1&Itemid=1
N2  - Lung adenocarcinoma (LAC) is a common and aggressive form of lung cancer that is increasing in incidence among never smokers at a younger age. Current treatment of patients with LAC is insufficient and there is a need for identification of effective biomarkers and development of therapeutic targets. These demands require also unproved models for in vivo and in vitro experimentation. In this study, we describe the establishment of two LAC cell lines, named LuCa-3 and LuCa-6. Both were derived from pleural effusion (PE) cells of LAC patients (13 and 16) and readily propagated as tumor xenografts in immunodeficient mice. PE cells from the patient L6 exhibited also the capacity for in vitro growth and were cultured in two forms: (i) as a suspension growing cell population, labeled LuCa-6S, composed of non-clumping single cells; and (ii) as a monolayer-like culture, labeled LuCa-6A, exhibiting tight cell-to-cell and to culture surface adherence. Unique features of these two sublines and their cell clones are the capacity to convert from a non-clumping single-cell suspension into the adherent growth pattern and vice versa. Immunostaining of patients' tumor tissue xenografts and cultured subline cells displayed markers specific for the phenotype of human LAC. LuCa-6S and LuCa-6A cells did not reveal a noticeable disparity in quantitative growth characteristics. However, a number of differences were detected between these two cell populations manifested in detection or intensities of antigen expressions on the cell surface (CD133, SFTPC) and in the nucleus (TTF-1) including pluripotcnt (OCT-4, SOX-2, NANOG) genes in cancer stem-like cells (CSCs). Dissimilarities between these two sublines were also detected in N-glycan profiles and in the sensitivity to natural killer cells. Salient features of these subline cell populations are responsiveness to selective upregulation of the pluripotent genes in subsets of CSCs via conversion of their growth patterns and/or by using culture stem media with growth factors. The described in vivo/in vitro model enables broader experimental approaches in studies of lung adenocarcinoma.
ER  -

POPOVIČ, Mikuláš, Yiling LIU, Erika LATTOVÁ, Dean MANN, Sabrina CURRELI, Zbyněk ZDRÁHAL, Martin EDELMAN and Joseph BRYANT. In vivo and in vitro cell-based model of lung adenocarcinoma from patients with pleural effusion. \textit{Neoplasma}. Slovenská akademie vied, 2021, vol.~68, No~3, p.~498-508. ISSN~0028-2685. Available from: https://dx.doi.org/10.4149/neo\_{}2021\_{}200906N953.

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