Determination of Renal Distribution of Zinc, Copper, Iron, and
Platinum in Mouse Kidney Using LA-ICP-MS

J 2021

Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS

ŠTĚPKA, Petr, Monika KRATOCHVÍLOVÁ, Michaela KUCHYNKA, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ et. al.

Basic information

Original name

Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS

Authors

ŠTĚPKA, Petr (203 Czech Republic, belonging to the institution), Monika KRATOCHVÍLOVÁ (203 Czech Republic, belonging to the institution), Michaela KUCHYNKA (203 Czech Republic, belonging to the institution), Martina RAUDENSKÁ (203 Czech Republic, belonging to the institution), Hana HOLCOVÁ POLANSKÁ (203 Czech Republic, belonging to the institution), Tomáš VIČAR (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution), Markéta VACULOVIČOVÁ (203 Czech Republic) and Michal MASAŘÍK (203 Czech Republic, guarantor, belonging to the institution)

Edition

BioMed Research International, London, Hindawi, 2021, 2314-6133

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30105 Physiology

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 3.246

RIV identification code

RIV/00216224:14110/21:00119606

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1155/2021/6800294

UT WoS

000783548000003

Keywords in English

zinc; copper; iron; platinum; renal distribution; mouse kidney; LA-ICP-MS

Abstract

V originále

The main dose-limiting side effect of cisplatin is nephrotoxicity. The utilization of cisplatin is an issue of balancing tumour toxicity versus platinum-induced nephrotoxicity. In this study, we focused on intraorgan distribution of common essential trace elements zinc, copper, and iron in healthy mouse kidneys and distribution of platinum after cisplatin treatment. Renal distribution in 12 nontreated Nu-Nu mice (males) was assessed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Furthermore, 9 Nu-Nu mice were treated with cisplatin. The order of elements concentration in kidneys was as follows: Fe > Zn > Cu. All three metals showed the higher concentrations at the cortex and medulla (28.60, 3.35, and 93.83 μg/g for Zn, Cu, and Fe, respectively) and lower concentration at the pelvis and the urinary tract (20.20, 1.93, and 62.48 μg/g for Zn, Cu, and Fe, respectively). No statistically significant difference between cortex and medulla was observed for these elements. After platinum treatment, the concentration of platinum in kidneys was enhanced more than 60-times, . Platinum significantly showed the highest accumulation in cortex (2.11 μg/g) with a gradient distribution. Platinum was less accumulated in medulla and pelvis than in cortex, and the lowest accumulation occurred in the urinary tract (1.13 μg/g). Image processing has been successfully utilized to colocalize metal distribution using LA-ICP-MS and histological samples images.

Links

GA20-02203S, research and development project

Name: Analýza tkáňové odpovědi na inhalaci nanočástic kovů a mechanismus jejich čištění

Investor: Czech Science Foundation

MUNI/A/1246/2020, interní kód MU

Name: Kardiovaskulární systém: od iontového kanálu k celotělovému modelu (Acronym: KAVASYKAMO)

Investor: Masaryk University

MUNI/A/1698/2020, interní kód MU

Name: Od molekulární, buněčné a tkáňové k systémové patofyziologii vybraných komplexních nemocí (Acronym: ComplexPF)

Investor: Masaryk University

Citovat

ŠTĚPKA, Petr, Monika KRATOCHVÍLOVÁ, Michaela KUCHYNKA, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ, Tomáš VIČAR, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ and Michal MASAŘÍK. Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS. BioMed Research International. London: Hindawi, 2021, vol. 2021, October 2021, p. 1-8. ISSN 2314-6133. Available from: https://dx.doi.org/10.1155/2021/6800294.

@article{1826217,
   author = {Štěpka, Petr and Kratochvílová, Monika and Kuchynka, Michaela and Raudenská, Martina and Holcová Polanská, Hana and Vičar, Tomáš and Vaculovič, Tomáš and Vaculovičová, Markéta and Masařík, Michal},
   article_location = {London},
   article_number = {October 2021},
   doi = {http://dx.doi.org/10.1155/2021/6800294},
   keywords = {zinc; copper; iron; platinum; renal distribution; mouse kidney; LA-ICP-MS},
   language = {eng},
   issn = {2314-6133},
   journal = {BioMed Research International},
   title = {Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS},
   url = {https://www.hindawi.com/journals/bmri/2021/6800294/#supplementary-materials},
   volume = {2021},
   year = {2021}
}

TY  - JOUR
ID  - 1826217
AU  - Štěpka, Petr - Kratochvílová, Monika - Kuchynka, Michaela - Raudenská, Martina - Holcová Polanská, Hana - Vičar, Tomáš - Vaculovič, Tomáš - Vaculovičová, Markéta - Masařík, Michal
PY  - 2021
TI  - Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS
JF  - BioMed Research International
VL  - 2021
IS  - October 2021
SP  - 1-8
EP  - 1-8
PB  - Hindawi
SN  - 23146133
KW  - zinc
KW  - copper
KW  - iron
KW  - platinum
KW  - renal distribution
KW  - mouse kidney
KW  - LA-ICP-MS
UR  - https://www.hindawi.com/journals/bmri/2021/6800294/#supplementary-materials
N2  - The main dose-limiting side effect of cisplatin is nephrotoxicity. The utilization of cisplatin is an issue of balancing tumour toxicity versus platinum-induced nephrotoxicity. In this study, we focused on intraorgan distribution of common essential trace elements zinc, copper, and iron in healthy mouse kidneys and distribution of platinum after cisplatin treatment. Renal distribution in 12 nontreated Nu-Nu mice (males) was assessed by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Furthermore, 9 Nu-Nu mice were treated with cisplatin. The order of elements concentration in kidneys was as follows: Fe > Zn > Cu. All three metals showed the higher concentrations at the cortex and medulla (28.60, 3.35, and 93.83 μg/g for Zn, Cu, and Fe, respectively) and lower concentration at the pelvis and the urinary tract (20.20, 1.93, and 62.48 μg/g for Zn, Cu, and Fe, respectively). No statistically significant difference between cortex and medulla was observed for these elements. After platinum treatment, the concentration of platinum in kidneys was enhanced more than 60-times, . Platinum significantly showed the highest accumulation in cortex (2.11 μg/g) with a gradient distribution. Platinum was less accumulated in medulla and pelvis than in cortex, and the lowest accumulation occurred in the urinary tract (1.13 μg/g). Image processing has been successfully utilized to colocalize metal distribution using LA-ICP-MS and histological samples images.
ER  -

ŠTĚPKA, Petr, Monika KRATOCHVÍLOVÁ, Michaela KUCHYNKA, Martina RAUDENSKÁ, Hana HOLCOVÁ POLANSKÁ, Tomáš VIČAR, Tomáš VACULOVIČ, Markéta VACULOVIČOVÁ and Michal MASAŘÍK. Determination of Renal Distribution of Zinc, Copper, Iron, and Platinum in Mouse Kidney Using LA-ICP-MS. \textit{BioMed Research International}. London: Hindawi, 2021, vol.~2021, October 2021, p.~1-8. ISSN~2314-6133. Available from: https://dx.doi.org/10.1155/2021/6800294.

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