| VLČNOVSKÁ, Marcela, Aneta ŠTOSSOVÁ, Michaela KUCHYNKA, Veronika FALTUSOVÁ, Hana HOLCOVÁ POLANSKÁ, Michal MASAŘÍK, Roman HRSTKA, Vojtech ADAM, Viktor KANICKÝ, Tomáš VACULOVIČ and Markéta VACULOVIČOVÁ. Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection. Molecules. Basel: MDPI, 2021, vol. 26, No 3, p. 1-11. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules26030630. |
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@article{1826225,
author = {Vlčnovská, Marcela and Štossová, Aneta and Kuchynka, Michaela and Faltusová, Veronika and Holcová Polanská, Hana and Masařík, Michal and Hrstka, Roman and Adam, Vojtech and Kanický, Viktor and Vaculovič, Tomáš and Vaculovičová, Markéta},
article_location = {Basel},
article_number = {3},
doi = {http://dx.doi.org/10.3390/molecules26030630},
keywords = {protein p53; dot-blot; antibody},
language = {eng},
issn = {1420-3049},
journal = {Molecules},
title = {Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection},
url = {https://www.mdpi.com/1420-3049/26/3/630},
volume = {26},
year = {2021}
}
TY - JOUR
ID - 1826225
AU - Vlčnovská, Marcela - Štossová, Aneta - Kuchynka, Michaela - Faltusová, Veronika - Holcová Polanská, Hana - Masařík, Michal - Hrstka, Roman - Adam, Vojtech - Kanický, Viktor - Vaculovič, Tomáš - Vaculovičová, Markéta
PY - 2021
TI - Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
JF - Molecules
VL - 26
IS - 3
SP - 1-11
EP - 1-11
PB - MDPI
SN - 14203049
KW - protein p53
KW - dot-blot
KW - antibody
UR - https://www.mdpi.com/1420-3049/26/3/630
N2 - Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.
ER -
VLČNOVSKÁ, Marcela, Aneta ŠTOSSOVÁ, Michaela KUCHYNKA, Veronika FALTUSOVÁ, Hana HOLCOVÁ POLANSKÁ, Michal MASAŘÍK, Roman HRSTKA, Vojtech ADAM, Viktor KANICKÝ, Tomáš VACULOVIČ and Markéta VACULOVIČOVÁ. Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection. \textit{Molecules}. Basel: MDPI, 2021, vol.~26, No~3, p.~1-11. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules26030630.
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