Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for
Immunoanalysis Using LA-ICP-MS Detection

J 2021

Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection

VLČNOVSKÁ, Marcela, Aneta ŠTOSSOVÁ, Michaela KUCHYNKA, Veronika FALTUSOVÁ, Hana HOLCOVÁ POLANSKÁ et. al.

Basic information

Original name

Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection

Authors

VLČNOVSKÁ, Marcela (203 Czech Republic, belonging to the institution), Aneta ŠTOSSOVÁ (203 Czech Republic, belonging to the institution), Michaela KUCHYNKA (203 Czech Republic, belonging to the institution), Veronika FALTUSOVÁ (703 Slovakia, belonging to the institution), Hana HOLCOVÁ POLANSKÁ (203 Czech Republic, belonging to the institution), Michal MASAŘÍK (203 Czech Republic, belonging to the institution), Roman HRSTKA (203 Czech Republic), Vojtech ADAM (203 Czech Republic), Viktor KANICKÝ (203 Czech Republic, belonging to the institution), Tomáš VACULOVIČ (203 Czech Republic, belonging to the institution) and Markéta VACULOVIČOVÁ (203 Czech Republic, guarantor)

Edition

Molecules, Basel, MDPI, 2021, 1420-3049

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 4.927

RIV identification code

RIV/00216224:14110/21:00119608

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.3390/molecules26030630

UT WoS

000615460400001

Keywords in English

protein p53; dot-blot; antibody

Abstract

V originále

Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.

Links

GA17-12774S, research and development project

Name: Využití vícenásobného značení pomocí kovových nanočástic pro bio-zobrazování

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

MUNI/A/1698/2020, interní kód MU

Name: Od molekulární, buněčné a tkáňové k systémové patofyziologii vybraných komplexních nemocí (Acronym: ComplexPF)

Investor: Masaryk University

Citovat

VLČNOVSKÁ, Marcela, Aneta ŠTOSSOVÁ, Michaela KUCHYNKA, Veronika FALTUSOVÁ, Hana HOLCOVÁ POLANSKÁ, Michal MASAŘÍK, Roman HRSTKA, Vojtech ADAM, Viktor KANICKÝ, Tomáš VACULOVIČ and Markéta VACULOVIČOVÁ. Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection. Molecules. Basel: MDPI, 2021, vol. 26, No 3, p. 1-11. ISSN 1420-3049. Available from: https://dx.doi.org/10.3390/molecules26030630.

@article{1826225,
   author = {Vlčnovská, Marcela and Štossová, Aneta and Kuchynka, Michaela and Faltusová, Veronika and Holcová Polanská, Hana and Masařík, Michal and Hrstka, Roman and Adam, Vojtech and Kanický, Viktor and Vaculovič, Tomáš and Vaculovičová, Markéta},
   article_location = {Basel},
   article_number = {3},
   doi = {http://dx.doi.org/10.3390/molecules26030630},
   keywords = {protein p53; dot-blot; antibody},
   language = {eng},
   issn = {1420-3049},
   journal = {Molecules},
   title = {Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection},
   url = {https://www.mdpi.com/1420-3049/26/3/630},
   volume = {26},
   year = {2021}
}

TY  - JOUR
ID  - 1826225
AU  - Vlčnovská, Marcela - Štossová, Aneta - Kuchynka, Michaela - Faltusová, Veronika - Holcová Polanská, Hana - Masařík, Michal - Hrstka, Roman - Adam, Vojtech - Kanický, Viktor - Vaculovič, Tomáš - Vaculovičová, Markéta
PY  - 2021
TI  - Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection
JF  - Molecules
VL  - 26
IS  - 3
SP  - 1-11
EP  - 1-11
PB  - MDPI
SN  - 14203049
KW  - protein p53
KW  - dot-blot
KW  - antibody
UR  - https://www.mdpi.com/1420-3049/26/3/630
N2  - Immunochemical methods are used not only in clinical practice for the diagnosis of a wide range of diseases but also in basic and advanced research. Based on the unique reaction between the antibody and its respective antigens, it serves to specifically recognize target molecules in biological complex samples. Current methods of labelling antibodies with elemental labels followed by detection by inductively coupled plasma mass spectrometry (ICP-MS) allow detection of multiple antigens in parallel in a single analysis. Using the laser ablation (LA) modality (LA-ICP-MS), it is also possible to monitor the spatial distribution of biogenic elements. Moreover, the employment of metal nanoparticle-labeled antibodies expands the applicability also to molecular imaging by LA-ICP-MS. In this work, conjugates of model monoclonal antibody (DO-1, recognizing p53 protein) with various metal nanoparticles-based labels were created and utilized in dot-blot analysis in order to compare their benefits and disadvantages. Based on experiments with the p53 protein standard, commercial kits of gold nanoparticles proved to be the most suitable for the preparation of conjugates. The LA-ICP-MS demonstrated very good repeatability, wide linear dynamic range (0.1-14 ng), and limit of detection was calculated as a 1.3 pg of p53 protein.
ER  -

VLČNOVSKÁ, Marcela, Aneta ŠTOSSOVÁ, Michaela KUCHYNKA, Veronika FALTUSOVÁ, Hana HOLCOVÁ POLANSKÁ, Michal MASAŘÍK, Roman HRSTKA, Vojtech ADAM, Viktor KANICKÝ, Tomáš VACULOVIČ and Markéta VACULOVIČOVÁ. Comparison of Metal Nanoparticles (Au, Ag, Eu, Cd) Used for Immunoanalysis Using LA-ICP-MS Detection. \textit{Molecules}. Basel: MDPI, 2021, vol.~26, No~3, p.~1-11. ISSN~1420-3049. Available from: https://dx.doi.org/10.3390/molecules26030630.

Detailed Information on Publication Record