Detailed Information on Publication Record
2021
Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
ILÍKOVÁ, Iva, Petr ILÍK, Monika OPATÍKOVÁ, Rameez ARSHAD, Lukáš NOSEK et. al.Basic information
Original name
Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
Authors
ILÍKOVÁ, Iva (203 Czech Republic), Petr ILÍK (203 Czech Republic), Monika OPATÍKOVÁ (203 Czech Republic), Rameez ARSHAD (528 Netherlands), Lukáš NOSEK (203 Czech Republic), Václav KARLICKÝ (203 Czech Republic), Zuzana KUČEROVÁ (203 Czech Republic), Pavel ROUDNICKÝ (203 Czech Republic, guarantor, belonging to the institution), Pavel POSPÍŠIL (203 Czech Republic), Dušan LAZÁR (203 Czech Republic), Jan BARTOŠ (203 Czech Republic) and Roman KOUŘIL (203 Czech Republic)
Edition
Plant Physiology, American Society of Plant Physiologists, 2021, 0032-0889
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10611 Plant sciences, botany
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
References:
Impact factor
Impact factor: 8.005
RIV identification code
RIV/00216224:14740/21:00123926
Organization unit
Central European Institute of Technology
UT WoS
000733403700059
Keywords in English
EXCITATION-ENERGY TRANSFERTHYLAKOID MEMBRANESCHLOROPHYLL FLUORESCENCEGRANA MEMBRANESCIRCULAR-DICHROISMELECTRON-TRANSPORTDYNAMIC REORGANIZATIONMACRO-ORGANIZATIONNORWAY SPRUCEMINOR ANTENNA
Tags
International impact, Reviewed
Změněno: 10/10/2024 14:49, Ing. Martina Blahová
Abstract
V originále
The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C-2), two strongly (S-2) and two moderately (M-2) bound light-harvesting protein (LHCB) trimers attached to C-2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure-function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 Ihcb6 to examine the possibility of the formation of the "spruce-type" PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The "spruce-type" PSII supercomplex was observed with low abundance only in the Ihcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2 semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2 arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.
Links
LM2018127, research and development project |
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90127, large research infrastructures |
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