Towards spruce-type photosystem II: consequences of the loss of
light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

J 2021

Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

ILÍKOVÁ, Iva, Petr ILÍK, Monika OPATÍKOVÁ, Rameez ARSHAD, Lukáš NOSEK et. al.

Basic information

Original name

Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis

Authors

ILÍKOVÁ, Iva (203 Czech Republic), Petr ILÍK (203 Czech Republic), Monika OPATÍKOVÁ (203 Czech Republic), Rameez ARSHAD (528 Netherlands), Lukáš NOSEK (203 Czech Republic), Václav KARLICKÝ (203 Czech Republic), Zuzana KUČEROVÁ (203 Czech Republic), Pavel ROUDNICKÝ (203 Czech Republic, guarantor, belonging to the institution), Pavel POSPÍŠIL (203 Czech Republic), Dušan LAZÁR (203 Czech Republic), Jan BARTOŠ (203 Czech Republic) and Roman KOUŘIL (203 Czech Republic)

Edition

Plant Physiology, American Society of Plant Physiologists, 2021, 0032-0889

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 8.005

RIV identification code

RIV/00216224:14740/21:00123926

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1093/plphys/kiab396

UT WoS

000733403700059

Keywords in English

EXCITATION-ENERGY TRANSFERTHYLAKOID MEMBRANESCHLOROPHYLL FLUORESCENCEGRANA MEMBRANESCIRCULAR-DICHROISMELECTRON-TRANSPORTDYNAMIC REORGANIZATIONMACRO-ORGANIZATIONNORWAY SPRUCEMINOR ANTENNA

Abstract

V originále

The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C-2), two strongly (S-2) and two moderately (M-2) bound light-harvesting protein (LHCB) trimers attached to C-2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure-function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 Ihcb6 to examine the possibility of the formation of the "spruce-type" PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The "spruce-type" PSII supercomplex was observed with low abundance only in the Ihcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2 semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2 arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

ILÍKOVÁ, Iva, Petr ILÍK, Monika OPATÍKOVÁ, Rameez ARSHAD, Lukáš NOSEK, Václav KARLICKÝ, Zuzana KUČEROVÁ, Pavel ROUDNICKÝ, Pavel POSPÍŠIL, Dušan LAZÁR, Jan BARTOŠ and Roman KOUŘIL. Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis. Plant Physiology. American Society of Plant Physiologists, 2021, vol. 187, No 4, p. 2691-2715. ISSN 0032-0889. Available from: https://dx.doi.org/10.1093/plphys/kiab396.

@article{1826359,
   author = {Ilíková, Iva and Ilík, Petr and Opatíková, Monika and Arshad, Rameez and Nosek, Lukáš and Karlický, Václav and Kučerová, Zuzana and Roudnický, Pavel and Pospíšil, Pavel and Lazár, Dušan and Bartoš, Jan and Kouřil, Roman},
   article_number = {4},
   doi = {http://dx.doi.org/10.1093/plphys/kiab396},
   keywords = {EXCITATION-ENERGY TRANSFERTHYLAKOID MEMBRANESCHLOROPHYLL FLUORESCENCEGRANA MEMBRANESCIRCULAR-DICHROISMELECTRON-TRANSPORTDYNAMIC REORGANIZATIONMACRO-ORGANIZATIONNORWAY SPRUCEMINOR ANTENNA},
   language = {eng},
   issn = {0032-0889},
   journal = {Plant Physiology},
   title = {Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis},
   url = {https://academic.oup.com/plphys/article/187/4/2691/6361658},
   volume = {187},
   year = {2021}
}

TY  - JOUR
ID  - 1826359
AU  - Ilíková, Iva - Ilík, Petr - Opatíková, Monika - Arshad, Rameez - Nosek, Lukáš - Karlický, Václav - Kučerová, Zuzana - Roudnický, Pavel - Pospíšil, Pavel - Lazár, Dušan - Bartoš, Jan - Kouřil, Roman
PY  - 2021
TI  - Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis
JF  - Plant Physiology
VL  - 187
IS  - 4
SP  - 2691-2715
EP  - 2691-2715
PB  - American Society of Plant Physiologists
SN  - 00320889
KW  - EXCITATION-ENERGY TRANSFERTHYLAKOID MEMBRANESCHLOROPHYLL FLUORESCENCEGRANA MEMBRANESCIRCULAR-DICHROISMELECTRON-TRANSPORTDYNAMIC REORGANIZATIONMACRO-ORGANIZATIONNORWAY SPRUCEMINOR ANTENNA
UR  - https://academic.oup.com/plphys/article/187/4/2691/6361658
N2  - The largest stable photosystem II (PSII) supercomplex in land plants (C2S2M2) consists of a core complex dimer (C-2), two strongly (S-2) and two moderately (M-2) bound light-harvesting protein (LHCB) trimers attached to C-2 via monomeric antenna proteins LHCB4-6. Recently, we have shown that LHCB3 and LHCB6, presumably essential for land plants, are missing in Norway spruce (Picea abies), which results in a unique structure of its C2S2M2 supercomplex. Here, we performed structure-function characterization of PSII supercomplexes in Arabidopsis (Arabidopsis thaliana) mutants lhcb3, lhcb6, and lhcb3 Ihcb6 to examine the possibility of the formation of the "spruce-type" PSII supercomplex in angiosperms. Unlike in spruce, in Arabidopsis both LHCB3 and LHCB6 are necessary for stable binding of the M trimer to PSII core. The "spruce-type" PSII supercomplex was observed with low abundance only in the Ihcb3 plants and its formation did not require the presence of LHCB4.3, the only LHCB4-type protein in spruce. Electron microscopy analysis of grana membranes revealed that the majority of PSII in lhcb6 and namely in lhcb3 lhcb6 mutants were arranged into C2S2 semi-crystalline arrays, some of which appeared to structurally restrict plastoquinone diffusion. Mutants without LHCB6 were characterized by fast induction of non-photochemical quenching and, on the contrary to the previous lhcb6 study, by only transient slowdown of electron transport between PSII and PSI. We hypothesize that these functional changes, associated with the arrangement of PSII into C2S2 arrays in thylakoids, may be important for the photoprotection of both PSI and PSII upon abrupt high-light exposure.
ER  -

ILÍKOVÁ, Iva, Petr ILÍK, Monika OPATÍKOVÁ, Rameez ARSHAD, Lukáš NOSEK, Václav KARLICKÝ, Zuzana KUČEROVÁ, Pavel ROUDNICKÝ, Pavel POSPÍŠIL, Dušan LAZÁR, Jan BARTOŠ and Roman KOUŘIL. Towards spruce-type photosystem II: consequences of the loss of light-harvesting proteins LHCB3 and LHCB6 in Arabidopsis. \textit{Plant Physiology}. American Society of Plant Physiologists, 2021, vol.~187, No~4, p.~2691-2715. ISSN~0032-0889. Available from: https://dx.doi.org/10.1093/plphys/kiab396.

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