Delineation of a novel environmental phylogroup of the genus
Acinetobacter encompassing Acinetobacter terrae sp. nov.,
Acinetobacter terrestris sp. nov. and three other tentative
species

J 2021

Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ et. al.

Basic information

Original name

Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species

Authors

NEMEC, Alexandr (203 Czech Republic), Lenka RADOLFOVÁ-KŘÍŽOVÁ (203 Czech Republic), Martina MAIXNEROVÁ (203 Czech Republic), Matěj NEMEC (203 Czech Republic), Petra ŠPANĚLOVÁ (203 Czech Republic), Renata ŠAFRÁNKOVÁ (203 Czech Republic), Ondrej ŠEDO (203 Czech Republic, guarantor, belonging to the institution), Bruno S. LOPES (826 United Kingdom of Great Britain and Northern Ireland) and Paul G. HIGGINS (276 Germany)

Edition

Systematic and Applied Microbiology, Urban & Fischer, 2021, 0723-2020

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10606 Microbiology

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 4.064

RIV identification code

RIV/00216224:14740/21:00123929

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1016/j.syapm.2021.126217

UT WoS

000680042300010

Keywords in English

Whole genome sequence; Core genome phylogeny; MALDI-TOF MS; Carbon source assimilation

Abstract

V originále

This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 degrees C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 degrees C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct Glade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282(v) (= CCM 8986(T) = CCUG 73811(T) = CNCTC 8082(T)) and ANC 4471(T) (= CCM 8985(T) = CCUG 73812(T) = CNCTC 8093(T)), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter Glade primarily associated with terrestrial ecosystems.

Links

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018140, research and development project

Name: e-Infrastruktura CZ (Acronym: e-INFRA CZ)

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ, Renata ŠAFRÁNKOVÁ, Ondrej ŠEDO, Bruno S. LOPES and Paul G. HIGGINS. Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species. Systematic and Applied Microbiology. Urban & Fischer, 2021, vol. 44, No 4, p. "126217", 9 pp. ISSN 0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2021.126217.

@article{1826397,
   author = {Nemec, Alexandr and RadolfováandKřížová, Lenka and Maixnerová, Martina and Nemec, Matěj and Španělová, Petra and Šafránková, Renata and Šedo, Ondrej and Lopes, Bruno S. and Higgins, Paul G.},
   article_number = {4},
   doi = {http://dx.doi.org/10.1016/j.syapm.2021.126217},
   keywords = {Whole genome sequence; Core genome phylogeny; MALDI-TOF MS; Carbon source assimilation},
   language = {eng},
   issn = {0723-2020},
   journal = {Systematic and Applied Microbiology},
   title = {Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species},
   url = {https://www.sciencedirect.com/science/article/pii/S0723202021000400?via%3Dihub},
   volume = {44},
   year = {2021}
}

TY  - JOUR
ID  - 1826397
AU  - Nemec, Alexandr - Radolfová-Křížová, Lenka - Maixnerová, Martina - Nemec, Matěj - Španělová, Petra - Šafránková, Renata - Šedo, Ondrej - Lopes, Bruno S. - Higgins, Paul G.
PY  - 2021
TI  - Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species
JF  - Systematic and Applied Microbiology
VL  - 44
IS  - 4
SP  - "126217"
EP  - "126217"
PB  - Urban & Fischer
SN  - 07232020
KW  - Whole genome sequence
KW  - Core genome phylogeny
KW  - MALDI-TOF MS
KW  - Carbon source assimilation
UR  - https://www.sciencedirect.com/science/article/pii/S0723202021000400?via%3Dihub
N2  - This study aimed to define the taxonomic position and structure of a novel, taxonomically unique group of 26 Acinetobacter strains, provisionally designated Taxon 24 (T24). The strains were recovered from soil and freshwater ecosystems (n = 21) or animals (n = 5) in Czechia, Scotland, Germany, the Netherlands and Turkey between 1993 and 2015. They were non-glucose-acidifying, nonhemolytic, nonproteolytic, growing at 32 degrees C and on acetate and ethanol as single carbon sources, but not on 4-hydroxybenzoate and mostly not at 37 degrees C. Their whole-genome sequences were 3.0-3.7 Mb in size, with GC contents of 39.8-41.3%. Based on core genome phylogenetic analysis, the 26 strains formed a distinct Glade within the genus Acinetobacter, with strongly supported subclades termed T24A (n = 11), T24B (n = 8), T24C (n = 2), T24D (n = 3) and T24E (n = 2). The internal genomic ANIb values for these subclades were >94.8%, while the ANIb values between them were <92.5%. The results of MALDI-TOF MS-based analyses agreed with this classification. The five subclades differed from each other in the results of one to six carbon source assimilation tests. Given the genomic and phenotypic distinctness, internal coherence, numbers of available strains and geographically diverse origin of T24A and T24B, we propose the names Acinetobacter terrae sp. nov. and Acinetobacter terrestris sp. nov. for these two taxa, respectively. The type strains are ANC 4282(v) (= CCM 8986(T) = CCUG 73811(T) = CNCTC 8082(T)) and ANC 4471(T) (= CCM 8985(T) = CCUG 73812(T) = CNCTC 8093(T)), respectively. We conclude that these two species together with the other T24 strains represent a widely dispersed Acinetobacter Glade primarily associated with terrestrial ecosystems.
ER  -

NEMEC, Alexandr, Lenka RADOLFOVÁ-KŘÍŽOVÁ, Martina MAIXNEROVÁ, Matěj NEMEC, Petra ŠPANĚLOVÁ, Renata ŠAFRÁNKOVÁ, Ondrej ŠEDO, Bruno S. LOPES and Paul G. HIGGINS. Delineation of a novel environmental phylogroup of the genus Acinetobacter encompassing Acinetobacter terrae sp. nov., Acinetobacter terrestris sp. nov. and three other tentative species. \textit{Systematic and Applied Microbiology}. Urban \&{} Fischer, 2021, vol.~44, No~4, p.~''126217'', 9 pp. ISSN~0723-2020. Available from: https://dx.doi.org/10.1016/j.syapm.2021.126217.

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