Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following
Heat Stress where They Regulate the Expression of a Similar Set
of Genes

J 2021

Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ et. al.

Basic information

Original name

Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes

Authors

WIESE, Anna J. (203 Czech Republic), Lenka STEINBACHOVÁ (203 Czech Republic), Ljudmilla TIMOFEJEVA (203 Czech Republic), Vojtěch ČERMÁK (203 Czech Republic), Božena KLODOVÁ (203 Czech Republic), Sri Ranjani GANJI (203 Czech Republic, belonging to the institution), Mariana LIMONES-MENDEZ (203 Czech Republic), Pavel BOKVAJ (203 Czech Republic), Said HAFIDH (203 Czech Republic), David POTĚŠIL (203 Czech Republic, guarantor, belonging to the institution) and David HONYS (203 Czech Republic)

Edition

International Journal of Molecular Sciences, Molecular Diversity Preservation International, 2021, 1661-6596

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10608 Biochemistry and molecular biology

Country of publisher

Switzerland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

fulltext

Impact factor

Impact factor: 2.598 in 2011

RIV identification code

RIV/00216224:14740/21:00123930

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.3390/ijms22020530

UT WoS

000611888100001

Keywords in English

bZIP; heat stress; Arabidopsis; 14– 3– 3; localization; transcriptomics

Abstract

V originále

Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14-3-3 proteins, and bZIP18 and bZIP52 were found to bind 14-3-3 epsilon, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14-3-3 epsilon interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.

Links

EF16_019/0000738, research and development project

Name: Centrum experimentální biologie rostlin

LM2018127, research and development project

Name: Česká infrastruktura pro integrativní strukturní biologii (Acronym: CIISB)

Investor: Ministry of Education, Youth and Sports of the CR

LM2018129, research and development project

Name: Národní infrastruktura pro biologické a medicínské zobrazování Czech-BioImaging

Investor: Ministry of Education, Youth and Sports of the CR

90127, large research infrastructures

Name: CIISB II

Citovat

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL and David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. International Journal of Molecular Sciences. Molecular Diversity Preservation International, 2021, vol. 22, No 2, p. "530", 24 pp. ISSN 1661-6596. Available from: https://dx.doi.org/10.3390/ijms22020530.

@article{1826439,
   author = {Wiese, Anna J. and Steinbachová, Lenka and Timofejeva, Ljudmilla and Čermák, Vojtěch and Klodová, Božena and Ganji, Sri Ranjani and LimonesandMendez, Mariana and Bokvaj, Pavel and Hafidh, Said and Potěšil, David and Honys, David},
   article_number = {2},
   doi = {http://dx.doi.org/10.3390/ijms22020530},
   keywords = {bZIP; heat stress; Arabidopsis; 14– 3– 3; localization; transcriptomics},
   language = {eng},
   issn = {1661-6596},
   journal = {International Journal of Molecular Sciences},
   title = {Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes},
   url = {https://www.mdpi.com/1422-0067/22/2/530},
   volume = {22},
   year = {2021}
}

TY  - JOUR
ID  - 1826439
AU  - Wiese, Anna J. - Steinbachová, Lenka - Timofejeva, Ljudmilla - Čermák, Vojtěch - Klodová, Božena - Ganji, Sri Ranjani - Limones-Mendez, Mariana - Bokvaj, Pavel - Hafidh, Said - Potěšil, David - Honys, David
PY  - 2021
TI  - Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes
JF  - International Journal of Molecular Sciences
VL  - 22
IS  - 2
SP  - "530"
EP  - "530"
PB  - Molecular Diversity Preservation International
SN  - 16616596
KW  - bZIP
KW  - heat stress
KW  - Arabidopsis
KW  - 14–
KW  - 3–
KW  - 3
KW  - localization
KW  - transcriptomics
UR  - https://www.mdpi.com/1422-0067/22/2/530
N2  - Heat stress (HS) is a major abiotic stress that negatively impacts crop yields across the globe. Plants respond to elevated temperatures by changing gene expression, mediated by transcription factors (TFs) functioning to enhance HS tolerance. The involvement of Group I bZIP TFs in the heat stress response (HSR) is not known. In this study, bZIP18 and bZIP52 were investigated for their possible role in the HSR. Localization experiments revealed their nuclear accumulation following heat stress, which was found to be triggered by dephosphorylation. Both TFs were found to possess two motifs containing serine residues that are candidates for phosphorylation. These motifs are recognized by 14-3-3 proteins, and bZIP18 and bZIP52 were found to bind 14-3-3 epsilon, the interaction of which sequesters them to the cytoplasm. Mutation of both residues abolished 14-3-3 epsilon interaction and led to a strict nuclear localization for both TFs. RNA-seq analysis revealed coordinated downregulation of several metabolic pathways including energy metabolism and translation, and upregulation of numerous lncRNAs in particular. These results support the idea that bZIP18 and bZIP52 are sequestered to the cytoplasm under control conditions, and that heat stress leads to their re-localization to nuclei, where they jointly regulate gene expression.
ER  -

WIESE, Anna J., Lenka STEINBACHOVÁ, Ljudmilla TIMOFEJEVA, Vojtěch ČERMÁK, Božena KLODOVÁ, Sri Ranjani GANJI, Mariana LIMONES-MENDEZ, Pavel BOKVAJ, Said HAFIDH, David POTĚŠIL and David HONYS. Arabidopsis bZIP18 and bZIP52 Accumulate in Nuclei Following Heat Stress where They Regulate the Expression of a Similar Set of Genes. \textit{International Journal of Molecular Sciences}. Molecular Diversity Preservation International, 2021, vol.~22, No~2, p.~''530'', 24 pp. ISSN~1661-6596. Available from: https://dx.doi.org/10.3390/ijms22020530.

Detailed Information on Publication Record