Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation

BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ and Pavel PLEVKA. Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation. In Phage/Virus Assembly 2021. 2021.

Basic information

• Original name: Baseplate structure of bacteriophage phi812 and mechanism of cell binding and degradation
• Authors: BÍŇOVSKÝ, Ján (703 Slovakia, belonging to the institution), Marta ŠIBOROVÁ (203 Czech Republic, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution), M. VAN RAAIJ and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Phage/Virus Assembly 2021, 2021.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10607 Virology
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14740/21:00123931
• Organization unit: Central European Institute of Technology
• Keywords in English: Staphylococcus aureus; Bacteriophage; Myoviridae
• Tags: rivok

Abstract

Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death . Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of S. aureus isolates and therefore is a promising candidate for a phage therapy agent . As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate, resulting in phage tail contraction and genome ejection . Mechanistic description of the baseplate re-organization, however, remains unknown. Using cryo-electron microscopy (cryo-EM), we reconstructed the phage baseplate in native and contracted states. The reconstruction of the center of native baseplate reaches resolution of 4 Å, which enables us to build individual protein structures. Also, selected proteins involved in host cell wall binding and penetration were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. The protein structures will be fitted into reconstruction of the contracted baseplate. Our results provide first structural characterisation of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail.

Links

• LL1906, research and development project: Name: Replikace fágů v bakteriálním biofilmu

Investor: Ministry of Education, Youth and Sports of the CR, Phage replication in bacterial biofilm