Baseplate structure of bacteriophage phi812 reveals mechanism
of cell wall binding and penetration

a 2021

Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration

BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ, Pavel PLEVKA et. al.

Basic information

Original name

Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration

Authors

BÍŇOVSKÝ, Ján (703 Slovakia, belonging to the institution), Marta ŠIBOROVÁ (203 Czech Republic, belonging to the institution), Jiří NOVÁČEK (203 Czech Republic, belonging to the institution), M. VAN RAAIJ and Pavel PLEVKA (203 Czech Republic, guarantor, belonging to the institution)

Edition

1st Student Conference by Czech Society for Structural Biology, 2021

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10607 Virology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

RIV identification code

RIV/00216224:14740/21:00123934

Organization unit

Central European Institute of Technology

Keywords in English

Staphylococcus aureus; Bacteriophage; Myoviridae

Abstract

V originále

Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death. Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of clinical isolates of S. aureus and therefore is a promising candidate for a phage therapy agent . As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate, resulting in phage tail contraction and genome ejection . Mechanistic description of the baseplate re-organization, however, remains unknown. Using cryo-electron microscopy (cryo-EM), we reconstructed the phage baseplate in native and contracted states (Fig. 1). The reconstruction of native baseplate reaches resolution of 4-5 Å and we are in process of building individual protein structures. Also, selected proteins involved in host cell wall attachment and degradation were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. The protein structures will be fitted into reconstruction of the contracted baseplate. Our results provide first structural characterisation of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail.

Links

LL1906, research and development project

Name: Replikace fágů v bakteriálním biofilmu

Investor: Ministry of Education, Youth and Sports of the CR, Phage replication in bacterial biofilm

Citovat

BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ and Pavel PLEVKA. Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration. In 1st Student Conference by Czech Society for Structural Biology. 2021.

@proceedings{1826499,
   author = {Bíňovský, Ján and Šiborová, Marta and Nováček, Jiří and Van Raaij, M. and Plevka, Pavel},
   booktitle = {1st Student Conference by Czech Society for Structural Biology},
   keywords = {Staphylococcus aureus; Bacteriophage; Myoviridae},
   language = {eng},
   title = {Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration},
   url = {https://cssb.structbio.org/wp-content/uploads/1stSCSB_booklet_fin.pdf},
   year = {2021}
}

TY  - CONF
ID  - 1826499
AU  - Bíňovský, Ján - Šiborová, Marta - Nováček, Jiří - Van Raaij, M. - Plevka, Pavel
PY  - 2021
TI  - Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration
KW  - Staphylococcus aureus
KW  - Bacteriophage
KW  - Myoviridae
UR  - https://cssb.structbio.org/wp-content/uploads/1stSCSB_booklet_fin.pdf
N2  - Antibiotic-resistant strains of Staphylococcus aureus cause human infections that are difficult to treat and can lead to death. Bacteriophage (phage) phi812K1/420 from the family Myoviridae infects 95% of clinical isolates of S. aureus and therefore is a promising candidate for a phage therapy agent . As the native phage particle approaches its host cell, phage receptor-binding proteins make a contact with the host cell wall. This interaction triggers a cascade of structural changes in the baseplate, resulting in phage tail contraction and genome ejection . Mechanistic description of the baseplate re-organization, however, remains unknown. Using cryo-electron microscopy (cryo-EM), we reconstructed the phage baseplate in native and contracted states (Fig. 1). The reconstruction of native baseplate reaches resolution of 4-5 Å and we are in process of building individual protein structures. Also, selected proteins involved in host cell wall attachment and degradation were produced in recombinant form and their structures were solved using X-ray crystallography and cryo-EM single-particle reconstruction. The protein structures will be fitted into reconstruction of the contracted baseplate. Our results provide first structural characterisation of contractile phage infecting a Gram-positive bacterium. Comparison of the two distinct baseplate states will allow us to describe molecular mechanism of initial stage of phage infection in detail.
ER  -

BÍŇOVSKÝ, Ján, Marta ŠIBOROVÁ, Jiří NOVÁČEK, M. VAN RAAIJ and Pavel PLEVKA. Baseplate structure of bacteriophage phi812 reveals mechanism of cell wall binding and penetration. In \textit{1st Student Conference by Czech Society for Structural Biology}. 2021.

Detailed Information on Publication Record