J 2021

Disruption of NAP1 genes in Arabidopsis thaliana suppresses the fas1 mutant phenotype, enhances genome stability and changes chromatin compaction

KOLÁŘOVÁ, Karolína, Martina NEŠPOR DADEJOVÁ, Tomáš LOJA, Gabriela LOCHMANOVÁ, Eva SÝKOROVÁ et. al.

Basic information

Original name

Disruption of NAP1 genes in Arabidopsis thaliana suppresses the fas1 mutant phenotype, enhances genome stability and changes chromatin compaction

Authors

KOLÁŘOVÁ, Karolína (203 Czech Republic, belonging to the institution), Martina NEŠPOR DADEJOVÁ (203 Czech Republic, belonging to the institution), Tomáš LOJA (703 Slovakia, belonging to the institution), Gabriela LOCHMANOVÁ (203 Czech Republic, belonging to the institution), Eva SÝKOROVÁ and Martina DVOŘÁČKOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

The Plant Journal, Blackwell Science, 2021, 0960-7412

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10611 Plant sciences, botany

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 7.091

RIV identification code

RIV/00216224:14740/21:00119611

Organization unit

Central European Institute of Technology

DOI

UT WoS

000620293700001

Keywords in English

chromatin; histone chaperones; histone variants; DNA repair

Tags

Tags

International impact, Reviewed
Změněno: 15/10/2024 14:19, Ing. Martina Blahová

Abstract

V originále

Histone chaperones mediate the assembly and disassembly of nucleosomes and participate in essentially all DNA-dependent cellular processes. In Arabidopsis thaliana, loss-of-function of FAS1 or FAS2 subunits of the H3-H4 histone chaperone complex CHROMATIN ASSEMBLY FACTOR 1 (CAF-1) has a dramatic effect on plant morphology, growth and overall fitness. CAF-1 dysfunction can lead to altered chromatin compaction, systematic loss of repetitive elements or increased DNA damage, clearly demonstrating its severity. How chromatin composition is maintained without functional CAF-1 remains elusive. Here we show that disruption of the H2A-H2B histone chaperone NUCLEOSOME ASSEMBLY PROTEIN 1 (NAP1) suppresses the FAS1 loss-of-function phenotype. The quadruple mutant fas1 nap1;1 nap1;2 nap1;3 shows wild-type growth, decreased sensitivity to genotoxic stress and suppression of telomere and 45S rDNA loss. Chromatin of fas1 nap1;1 nap1;2 nap1;3 plants is less accessible to micrococcal nuclease and the nuclear H3.1 and H3.3 histone pools change compared to fas1. Consistently, association between NAP1 and H3 occurs in the cytoplasm and nucleus in vivo in protoplasts. Altogether we show that NAP1 proteins play an essential role in DNA repair in fas1, which is coupled to nucleosome assembly through modulation of H3 levels in the nucleus.

Links

EF16_026/0008446, research and development project
Name: Integrace signálu a epigenetické reprogramování pro produktivitu rostlin
GA18-07027S, research and development project
Name: Zapojení telomerázy do buněčného interaktomu
Investor: Czech Science Foundation
GJ19-11880Y, research and development project
Name: Chromatinové prostředí indukované genotoxickými látkami
Investor: Czech Science Foundation, Chromatin environment induced by genotoxic agents
LQ1601, research and development project
Name: CEITEC 2020 (Acronym: CEITEC2020)
Investor: Ministry of Education, Youth and Sports of the CR
LTC18048, research and development project
Name: Prostorové uspořádání významných jaderných domén a jejich proteinových složek
Investor: Ministry of Education, Youth and Sports of the CR, Spatial organisation of key nuclear domains and their protein components, INTER-COST
90062, large research infrastructures
Name: Czech-BioImaging
90127, large research infrastructures
Name: CIISB II
90132, large research infrastructures
Name: NCMG II