A study of the effect of DAMPs on choroid plexus using an in-vitro model

LAKATOSOVÁ, Klaudia, Alemeh ZAMANI and Marek JOUKAL. A study of the effect of DAMPs on choroid plexus using an in-vitro model. In Morphology 2021. 2021.

Basic information

• Original name: A study of the effect of DAMPs on choroid plexus using an in-vitro model
• Name (in English): A study of the effect of DAMPs on choroid plexus using an in-vitro model
• Authors: LAKATOSOVÁ, Klaudia, Alemeh ZAMANI and Marek JOUKAL.
• Edition: Morphology 2021, 2021.

Other information

• Type of outcome: Presentations at conferences
• Confidentiality degree: is not subject to a state or trade secret

Abstract (in English)

In case of tissue injury, damage-associated molecular patterns (DAMPs) are released into the blood circulation. DAMPs might migrate through the blood-cerebrospinal fluid barrier (BCSF-B) and trigger toll-like receptors (TLRs) in the choroid plexus, which can potentially result in the immune reaction in the central nervous system. To study this mechanism an in-vitro model of choroid plexus (Z310 cells) was used and the proinflammatory reaction of choroidal epithelial cells in response to DAMPs was examined. Z310 cells represent primary choroidal epithelial cell culture established from rat choroid plexus tissue. Z310 cells were incubated with the pattern recognition receptors (PRRs) agonists, such as LPS (lipopolysaccharide), N-Formylated Peptide (fmlp), and CpG oligodeoxynucleotides (CPG ODN) for 24 hours. Cells were then used for immunocytochemistry and western blot analyses of expression of TLR4, TLR9, and cytokines IL-6, IL1-β, TNF-α. The expression of cytokines IL-6, IL1-β, TNF-α were upregulated in Z310 cells, following activation of PRRs via DAMPs. After tissue injury, DAMPs can migrate through BSCF-B and they have the ability to increase the pro-inflammatory profiling of the choroidal epithelial cells.

Links

• MUNI/A/1520/2020, interní kód MU: Name: Změny ve strukturách nervové soustavy v reakci na poškození

Investor: Masaryk University