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PEKARKOVA, Jana, Imrich GABLECH, Tatiana FIALOVA, Ondrej BILEK and Zdenka FOHLEROVÁ. Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability. Frontiers in bioengineering and biotechnology. Laussane: Frontiers Media S.A., 2021, vol. 9, December 2021, p. 1-11. ISSN 2296-4185. Available from: https://dx.doi.org/10.3389/fbioe.2021.782799.

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Basic information
Original name	Modifications of Parylene by Microstructures and Selenium Nanoparticles: Evaluation of Bacterial and Mesenchymal Stem Cell Viability
Authors	PEKARKOVA, Jana, Imrich GABLECH (203 Czech Republic), Tatiana FIALOVA (203 Czech Republic), Ondrej BILEK (203 Czech Republic) and Zdenka FOHLEROVÁ (203 Czech Republic, guarantor, belonging to the institution).
Edition	Frontiers in bioengineering and biotechnology, Laussane, Frontiers Media S.A. 2021, 2296-4185.

Other information
Original language	English
Type of outcome	Article in a journal
Field of Study	10608 Biochemistry and molecular biology
Country of publisher	Switzerland
Confidentiality degree	is not subject to a state or trade secret
WWW	URL
Impact factor	Impact factor: 6.064
RIV identification code	RIV/00216224:14110/21:00123976
Organization unit	Faculty of Medicine
Doi	http://dx.doi.org/10.3389/fbioe.2021.782799
UT WoS	000731445100001
Keywords in English	parylene-C; micropillars; selenium nanoparticles; biocompatibility; antimicrobial
Tags	14110512, rivok
Tags	International impact, Reviewed
Changed by	Changed by: Mgr. Tereza Miškechová, učo 341652. Changed: 27/1/2022 13:52.

Abstract
Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.

Abstract

Parylene-based implants or coatings introduce surfaces suffering from bacteria colonization. Here, we synthesized polyvinylpyrrolidone-stabilized selenium nanoparticles (SeNPs) as the antibacterial agent, and various approaches are studied for their reproducible adsorption, and thus the modification of parylene-C-coated glass substrate. The nanoparticle deposition process is optimized in the nanoparticle concentration to obtain evenly distributed NPs on the flat parylene-C surface. Moreover, the array of parylene-C micropillars is fabricated by the plasma etching of parylene-C on a silicon wafer, and the surface is modified with SeNPs. All designed surfaces are tested against two bacterial pathogens, Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). The results show no antibacterial effect toward S. aureus, while some bacteriostatic effect is observed for E. coli on the flat and microstructured parylene. However, SeNPs did not enhance the antibacterial effect against both bacteria. Additionally, all designed surfaces show cytotoxic effects toward mesenchymal stem cells at high SeNP deposition. These results provide valuable information about the potential antibacterial treatment of widely used parylene-C in biomedicine.