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@article{1829382,
author = {Gabbasov, Z. A. and Avtaeva, Y. N. and Melnikov, I. S. and Okhota, S. D. and Caprnda, M. and Mozos, I. and Prosecký, Robert and Rodrigo, L. and Kružliak, Peter and Zozulya, N. I.},
article_location = {HOBOKEN},
article_number = {9},
doi = {http://dx.doi.org/10.1002/jcla.23939},
keywords = {fibrinogen-coated surface; flow conditions; platelet adhesion; recording of a scattered laser light signal from a fibrinogen-covered surface; whole blood},
language = {eng},
issn = {0887-8013},
journal = {JOURNAL OF CLINICAL LABORATORY ANALYSIS},
title = {Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions},
url = {https://onlinelibrary.wiley.com/doi/10.1002/jcla.23939},
volume = {35},
year = {2021}
}
TY - JOUR
ID - 1829382
AU - Gabbasov, Z. A. - Avtaeva, Y. N. - Melnikov, I. S. - Okhota, S. D. - Caprnda, M. - Mozos, I. - Prosecký, Robert - Rodrigo, L. - Kružliak, Peter - Zozulya, N. I.
PY - 2021
TI - Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions
JF - JOURNAL OF CLINICAL LABORATORY ANALYSIS
VL - 35
IS - 9
SP - 1-9
EP - 1-9
PB - WILEY
SN - 08878013
KW - fibrinogen-coated surface
KW - flow conditions
KW - platelet adhesion
KW - recording of a scattered laser light signal from a fibrinogen-covered surface
KW - whole blood
UR - https://onlinelibrary.wiley.com/doi/10.1002/jcla.23939
N2 - Aim To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. Methods We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. Results The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 +/- 0.08V, n = 7) than in PRP (0.05 +/- 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Conclusion Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.
ER -
GABBASOV, Z. A., Y. N. AVTAEVA, I. S. MELNIKOV, S. D. OKHOTA, M. CAPRNDA, I. MOZOS, Robert PROSECKÝ, L. RODRIGO, Peter KRUŽLIAK and N. I. ZOZULYA. Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. \textit{JOURNAL OF CLINICAL LABORATORY ANALYSIS}. HOBOKEN: WILEY, 2021, vol.~35, No~9, p.~1-9. ISSN~0887-8013. Available from: https://dx.doi.org/10.1002/jcla.23939.
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