J 2021

Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions

GABBASOV, Z. A., Y. N. AVTAEVA, I. S. MELNIKOV, S. D. OKHOTA, M. CAPRNDA et. al.

Basic information

Original name

Kinetics of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions

Authors

GABBASOV, Z. A., Y. N. AVTAEVA, I. S. MELNIKOV, S. D. OKHOTA, M. CAPRNDA, I. MOZOS, Robert PROSECKÝ (203 Czech Republic, belonging to the institution), L. RODRIGO, Peter KRUŽLIAK (703 Slovakia, guarantor, belonging to the institution) and N. I. ZOZULYA

Edition

JOURNAL OF CLINICAL LABORATORY ANALYSIS, HOBOKEN, WILEY, 2021, 0887-8013

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

20602 Medical laboratory technology ;

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.124

RIV identification code

RIV/00216224:14110/21:00123997

Organization unit

Faculty of Medicine

DOI

UT WoS

000680966900001

Keywords in English

fibrinogen-coated surface; flow conditions; platelet adhesion; recording of a scattered laser light signal from a fibrinogen-covered surface; whole blood

Tags

Tags

International impact, Reviewed
Změněno: 31/1/2022 10:17, Mgr. Tereza Miškechová

Abstract

V originále

Aim To test a novel method of assessment of platelet adhesion to a fibrinogen-coated surface in whole blood under flow conditions. Methods We developed a fluidic device that mimics blood flow in vessels. The method of detection of platelet adhesion is based on recording of a scattered laser light signal from a fibrinogen-covered surface. Testing was performed in platelet-rich plasma (PRP) and whole blood of healthy volunteers. Control measurements were performed, followed by tests with inhibition of platelet GPIIa/IIIb and GPIb receptors. Then, the same testing sequence was performed in whole blood of persons with autoimmune thrombocytopenia and type 3 von Willebrand disease. Results The change in intensity of scattered light was 2.7 (2.4; 4.1) times higher in whole blood (0.2 +/- 0.08V, n = 7) than in PRP (0.05 +/- 0.02 V, n = 7), p < 0.01. The blocking of GP IIb/IIIa receptors decreased the intensity of scattered light to 8.5 (6.5;12)%; the blocking of GPIb receptors decreased it to 34 (23;58)%, p < 0.01. In the whole blood of a person with autoimmune thrombocytopenia, the inhibition of GPIb receptors decreased platelet adhesion, but no effect was observed in type 3 von Willebrand disease. Inhibition of platelet GPIIb/IIIa receptors alone or combined inhibition of GPIb and GPIIb/IIIa receptors resulted in almost total suppression of adhesion in both cases. Conclusion Our system effectively registers platelet adhesion to a fibrinogen-coated surface under controlled-flow conditions and may successfully be applied to the investigation of platelet adhesion kinetics.